CAJ | 학술논문

在生理条件下，用荧光光谱法研究了氯诺昔康对牛血清白蛋白，铜(Ⅱ)对牛血清白蛋白以及铜(Ⅱ)对氯诺昔康和牛血清白蛋白荧光光谱特性的影响。铜(Ⅱ)和氯诺昔康均可使牛血清白蛋白的荧光强度发生猝灭，在铜(Ⅱ)存在下,氯诺昔康对牛血清白蛋白的荧光猝灭作用显著增强。根据荧光猝灭双倒数图计算氯诺昔康和牛血清白蛋白之间的结合常数是5.43×105，结合位点数是1.14。铜(Ⅱ)和牛血清白蛋白之间的结合常数是2.10×105，结合位点数是0.78。
재생리조건하，용형광광보법연구료록낙석강대우혈청백단백，동(Ⅱ)대우혈청백단백이급동(Ⅱ)대록낙석강화우혈청백단백형광광보특성적영향。동(Ⅱ)화록낙석강균가사우혈청백단백적형광강도발생졸멸，재동(Ⅱ)존재하,록낙석강대우혈청백단백적형광졸멸작용현저증강。근거형광졸멸쌍도수도계산록낙석강화우혈청백단백지간적결합상수시5.43×105，결합위점수시1.14。동(Ⅱ)화우혈청백단백지간적결합상수시2.10×105，결합위점수시0.78。
Under the physiological conditions, fluorescence spectrometry was used to study bovine serum albumin, copper ( Ⅱ ) of bovine serum albumin and copper ( Ⅱ ) on bovine serum albumin fluorescence spectrum characteristics. Copper ( Ⅱ ) and lornoxicam could both cause bovine serum albumin fluorescence quenched. Under the existence of copper ( Ⅱ ), lornoxicam enhanced the fluorescence quenching effect of bovine serum albumin. According to lineweaver-burk plot calculation, the combining constant between lornoxicam and bovine serum albumin was 5.43×105, position points were 1.14. The combining constant between Copper (Ⅱ) and bovine serum albumin was 2.10×105, position points were 0.78.