CAJ | 학술논문

目的：通过不同浓度白头翁水煎液对离体蛙心功能的影响及其机制的研究，为新药的研制和深入临床实践提供理论依据。方法：采用斯氏蛙心灌流法制备离体蛙心标本，分别加入不同浓度(100%，50%，25%，12.5%）的白头翁水煎液(0.1 mL），观察药物对离体蛙心的作用，并了解是否存在剂量依赖性。加入0.05 mL、0.1 mg/mL盐酸维拉帕米溶液，观察并记录实验数据，然后加入白头翁水煎液，观察药物对离体蛙心的作用；加入0.05 mL、1 mg/mL盐酸普罗帕酮溶液，观察并记录实验数据，然后加入白头翁水煎液，观察药物对离体蛙心的作用。探讨其作用机制与钠、钙通道的关系。结果：4种不同浓度的白头翁水煎液均能有效地增强蛙心的收缩力，使蛙心收缩频率减慢。同时白头翁水煎液能逆转盐酸普罗帕酮对心脏的影响，但不能逆转盐酸维拉帕米对心脏的作用。结论：白头翁水煎液对离体蛙心有明显的影响，能逆转盐酸普罗帕酮溶液对心脏的作用。
목적：통과불동농도백두옹수전액대리체와심공능적영향급기궤제적연구，위신약적연제화심입림상실천제공이론의거。방법：채용사씨와심관류법제비리체와심표본，분별가입불동농도(100%，50%，25%，12.5%）적백두옹수전액(0.1 mL），관찰약물대리체와심적작용，병료해시부존재제량의뢰성。가입0.05 mL、0.1 mg/mL염산유랍파미용액，관찰병기록실험수거，연후가입백두옹수전액，관찰약물대리체와심적작용；가입0.05 mL、1 mg/mL염산보라파동용액，관찰병기록실험수거，연후가입백두옹수전액，관찰약물대리체와심적작용。탐토기작용궤제여납、개통도적관계。결과：4충불동농도적백두옹수전액균능유효지증강와심적수축력，사와심수축빈솔감만。동시백두옹수전액능역전염산보라파동대심장적영향，단불능역전염산유랍파미대심장적작용。결론：백두옹수전액대리체와심유명현적영향，능역전염산보라파동용액대심장적작용。
Objective:To provide theoretical basis for the development of new drug and clinical practice by in-vestigating the effects of BaiTouWeng (radix pulsatilla) decoction in different concentrations on isolated frog heart and the mechanism. Methods:The specimens of isolated frog hearts were established by the method of frog heart perfusion, the effects of different concentrations of BaiTouWeng decoction (100%, 50%, 25%and 12.5%) on isolated frog hearts were observed respectively, to investigate whether the dose-dependent manner was existed. The speci-mens were administered with verapamil hydrochloride, 0.05 mL, 0.1 mg/mL, experimental data was recorded and observed, BaiTouWeng decoction was added to survey its effects on isolated frog heart;the specimens were given with propafenone hydrochloride, 0.05 mL, 0.1 mg/mL, to record and observe experimental data, BaiTouWeng decoc-tion was added to survey its effects on isolated frog heart, the relationship among the mechanism, sodium and calci-um channel was explored. Results: BaiTouWeng decoction with different concentrations could effectively enhance cardiac contractility of the frogs, at the same time slow down contraction frequency of frog heart. It could also re-verse the effects of propafenone hydrochloride on frog heart, but showed no effects of verapamil hydrochloride influ-encing on the heart. Conclusion:BaiTouWeng decoction demonstrates obvious action on isolated frog heart and re-verses the influence of propafenone hydrochloride on the heart.