食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
4期
1073-1080
,共8页
张舒亚%张坤%樊彦莉%薛华杰%印丽萍%陈颖%张璜
張舒亞%張坤%樊彥莉%薛華傑%印麗萍%陳穎%張璜
장서아%장곤%번언리%설화걸%인려평%진영%장황
食品过敏原%羽扇豆%环介导等温扩增%检测
食品過敏原%羽扇豆%環介導等溫擴增%檢測
식품과민원%우선두%배개도등온확증%검측
food allergen%lupin%loop-mediated isothermal amplification%detection
目的：建立食品过敏原羽扇豆成分的环介导等温扩增检测方法(loop-mediated isothermal amplification, LAMP)。方法根据羽扇豆的 ITS 基因设计羽扇豆的特异性引物,进行特异度、灵敏度、稳定性测试,建立LAMP检测方法。结果本文建立的食品过敏原羽扇豆成分LAMP检测方法能有效对羽扇豆成分进行快速检测,具有较强的特异度和稳定性,灵敏度可达0.001%(w：w)。结论该方法特异度强、灵敏度高,可以快速、准确检测食品中过敏原羽扇豆成分。
目的：建立食品過敏原羽扇豆成分的環介導等溫擴增檢測方法(loop-mediated isothermal amplification, LAMP)。方法根據羽扇豆的 ITS 基因設計羽扇豆的特異性引物,進行特異度、靈敏度、穩定性測試,建立LAMP檢測方法。結果本文建立的食品過敏原羽扇豆成分LAMP檢測方法能有效對羽扇豆成分進行快速檢測,具有較彊的特異度和穩定性,靈敏度可達0.001%(w：w)。結論該方法特異度彊、靈敏度高,可以快速、準確檢測食品中過敏原羽扇豆成分。
목적：건립식품과민원우선두성분적배개도등온확증검측방법(loop-mediated isothermal amplification, LAMP)。방법근거우선두적 ITS 기인설계우선두적특이성인물,진행특이도、령민도、은정성측시,건립LAMP검측방법。결과본문건립적식품과민원우선두성분LAMP검측방법능유효대우선두성분진행쾌속검측,구유교강적특이도화은정성,령민도가체0.001%(w：w)。결론해방법특이도강、령민도고,가이쾌속、준학검측식품중과민원우선두성분。
Objective To develop a loop-mediated isothermal amplification(LAMP) for detection of allergen lupin components. Methods The primers were designed according to the ITS gene sequence and specificity, and sensitivity and stability of the LAMP detection method were analyzed. Results The de-tection method was specific and accurate, and the limit of detection was 0.001%(w:w). Conclusion The LAMP method is effective, specific and sensitive, and it is suitable for the detection of allergen lupin components in food.
