CAJ | 학술논문

目的：研究不同的大孔树脂对萱藻多酚的纯化效果，建立其纯化方法。方法以透明质酸酶抑制活性为抗过敏活性的评价指标，通过分析7种不同大孔树脂对萱藻多酚的静态和动态吸附曲线，建立并优化萱藻多酚的纯化工艺。结果 XDA-1型大孔树脂对萱藻多酚有良好的吸附性能和解吸效果，优化pH及解吸剂浓度后，通过一步大孔树脂纯化能够得到纯度达45.27%的萱藻多酚，其透明质酸酶抑制活性比纯化前提高了1.94倍。结论建立了萱藻多酚的大孔树脂一步法纯化方法。
목적：연구불동적대공수지대훤조다분적순화효과，건립기순화방법。방법이투명질산매억제활성위항과민활성적평개지표，통과분석7충불동대공수지대훤조다분적정태화동태흡부곡선，건립병우화훤조다분적순화공예。결과 XDA-1형대공수지대훤조다분유량호적흡부성능화해흡효과，우화pH급해흡제농도후，통과일보대공수지순화능구득도순도체45.27%적훤조다분，기투명질산매억제활성비순화전제고료1.94배。결론건립료훤조다분적대공수지일보법순화방법。
Objective To select appropriate macroporous resin for preliminary purification of polyphenols from Scytosiphon lomentsrius. Methods Seven different macroporous resins were evaluated with static and dynamic adsorption curves, and pH of sample, concentration of resolving agent were assessed to optimize the purification process. Results XDA-1 macroporous resins have good adsorption and desorption with poly-phenols. With the optimized process such as pH and resolving agents, polyphenols in Scytosiphon lomentsrius can be got with a purity of 45.27%. Compared with the crude extracts of Scytosiphon lomentsrius, the specific inhibition of hyaluronidase activity improved 1.94 times. Conclusion One step method for purification of polyphenols from Scytosiphon lomentsrius was established.