CAJ | 학술논문

目的：研究果胶是否能够降低Balb/c小鼠对OVA(鸡卵清白蛋白)的过敏反应水平,以及同时经口给予双歧杆菌是否能够调整小鼠肠道菌群,从而影响过敏反应的发生和反应强度。方法连续40 d经口给予小鼠20μg/只(低剂量)和200μg/只(高剂量)的果胶溶液或溶有双歧杆菌(2×106 CFU/只)的果胶溶液,每10 d腹腔注射OVA和铝佐剂致敏小鼠,10 d后进行大刺激,采血测定组胺、肥大细胞蛋白酶-1、免疫球蛋白E水平,并对脾脏T细胞进行体外刺激,检测IFN-γ和IL-4的水平,同时取小鼠粪便筛选双歧杆菌培养。结果果胶处理组小鼠血浆中组胺、肥大细胞蛋白酶-1水平与OVA致敏组相比无显著差异(P>0.05)；高剂量处理组小鼠血清总IgE水平显著低于OVA致敏组(P<0.05),特异性IgE及细胞因子水平无显著差异(P>0.05)；双歧杆菌筛选培养可以看出,果胶有增加双歧杆菌数量的作用,但经口给予双歧杆菌活菌无显著作用。结论补充果胶不能降低Balb/c小鼠的肥大细胞脱颗粒情况、抗体水平以及细胞因子产生水平；果胶有增加肠道内双歧杆菌的作用,但经口给予活菌不能增加肠道内双歧杆菌水平。
목적：연구과효시부능구강저Balb/c소서대OVA(계란청백단백)적과민반응수평,이급동시경구급여쌍기간균시부능구조정소서장도균군,종이영향과민반응적발생화반응강도。방법련속40 d경구급여소서20μg/지(저제량)화200μg/지(고제량)적과효용액혹용유쌍기간균(2×106 CFU/지)적과효용액,매10 d복강주사OVA화려좌제치민소서,10 d후진행대자격,채혈측정조알、비대세포단백매-1、면역구단백E수평,병대비장T세포진행체외자격,검측IFN-γ화IL-4적수평,동시취소서분편사선쌍기간균배양。결과과효처리조소서혈장중조알、비대세포단백매-1수평여OVA치민조상비무현저차이(P>0.05)；고제량처리조소서혈청총IgE수평현저저우OVA치민조(P<0.05),특이성IgE급세포인자수평무현저차이(P>0.05)；쌍기간균사선배양가이간출,과효유증가쌍기간균수량적작용,단경구급여쌍기간균활균무현저작용。결론보충과효불능강저Balb/c소서적비대세포탈과립정황、항체수평이급세포인자산생수평；과효유증가장도내쌍기간균적작용,단경구급여활균불능증가장도내쌍기간균수평。
Objective To study whether pectin can decrease the level of allergic reactions to ovalbumin ( OVA ) in Balb/c mice, and whether oral administration of bifidobacterium at the same time can adjust the in-testinal flora in mice to affect the level of an allergic reaction. Methods 20 μg/mouse (low dose) and 200μg/mouse (high dose) soluble pectin solution with/without Bifidobacterium (2×106 CFU/mouse) was given for 40 d, and OVA added alum adjuvant was intraperitoneally injected every 10 days to sensitize mice, then mice were stimulated with OVA 10 d later. The levels of plasma histamine, mast cells protease-1 and immunoglobu-lin E were determined by ELISA. Spleen T cells were prepared and stimulated in vitro to produce cytokines, and the levels of IFN-gamma and IL-4 were then measured. Fecal samples were collected to culture Bifidobac-terium, and compare the differences between groups. Results The levels of histamine, mast cells protease-1 level of mice administrated with pectin and mice sensitized by OVA showed no significant difference. Levels of serum total IgE in mice of high dose treatment were significantly lower than mice sensitized by OVA, while the levels of specific IgE and cytokines showed no significant difference. Pectin could increase the number of bifidobacteria, but oral administration of bifidobacterium viable showed no significant effect. Conclusion Levels of mast cell degranulation, antibodies and cytokines were not reduced by pectin in Balb/c mice. Pectin increased the number of intestinal Bifidobacterium, but it showed no obvious effect by given live bacteria.