CAJ | 학술논문

目的：建立食品过敏原牛奶成分LAMP(loop-mediated isothermal amplification)检测方法，并与实时荧光PCR(real-time PCR)检测方法比对。方法针对牛线粒体细胞色素b(cyt-b)基因设计LAMP引物并建立反应体系，在特异性和灵敏度方面与real-time PCR检测方法比对。结果本研究建立的LAMP方法检测9份不同品牌的牛奶和羊奶及其加工制品，没有出现交叉反应，具有良好的特异性。该方法的检测灵敏度为0.5%，与real-time PCR方法检测灵敏度相当。检测了69份实际样品，检测结果与real-time PCR检测结果一致。结论本研究建立的食品过敏原牛奶成分LAMP检测方法简单经济，检测结果可靠，可有效缩短检测时间，适用于过敏原牛奶成分的检测，具有良好的应用前景。
목적：건립식품과민원우내성분LAMP(loop-mediated isothermal amplification)검측방법，병여실시형광PCR(real-time PCR)검측방법비대。방법침대우선립체세포색소b(cyt-b)기인설계LAMP인물병건립반응체계，재특이성화령민도방면여real-time PCR검측방법비대。결과본연구건립적LAMP방법검측9빈불동품패적우내화양내급기가공제품，몰유출현교차반응，구유량호적특이성。해방법적검측령민도위0.5%，여real-time PCR방법검측령민도상당。검측료69빈실제양품，검측결과여real-time PCR검측결과일치。결론본연구건립적식품과민원우내성분LAMP검측방법간단경제，검측결과가고，가유효축단검측시간，괄용우과민원우내성분적검측，구유량호적응용전경。
Objective The loop-mediated isothermal amplification (LAMP) detection method was established for milk allergen testing and compared with the real-time PCR detection methods. Methods The cyt-b gene of bovine was used to design LAMP primers and then establish reaction system. The spe-cificity and sensitivity of LAMP were compared with real-time PCR detection method. Results The specificity of LAMP method was tested by 9 different milk and goat milk products. The results showed that the LAMP method was highly specific to milk. No cross-reaction was founded. The detection limit of LAMP reached 0.5%in base-material addition test which was consistent with the real-time PCR method. Through 69 practical food samples testing, the LAMP results were consistent with real-time PCR results. Conclusion The LAMP detection method of milk allergen established in this study is simple, economi-cal and reliable, which can effectively reduce the detection time and apply to the detection of milk aller-gen with good application prospects.