CAJ | 학술논문

目的：检测宫颈不同级别病变组织中人乳头瘤病毒16 E6（HPV16 E6）及人半翼基因（hWAPL）的表达，分析宫颈癌与HPV16 E6及hWAPL蛋白的相关性，为早期诊断宫颈癌提供依据。方法采用免疫组化方法检测宫颈不同级别病变组织的石蜡包埋块，其中宫颈上皮细胞内瘤变（CIN）Ⅰ或CIN Ⅱ49例，CIN Ⅲ或原位癌33例，宫颈浸润癌（ICC）28例，正常宫颈上皮组织16例。结果 HPV16E6与hWAPL在正常宫颈组、CINⅠ或Ⅱ组、CINⅢ组和ICC组的组织中阳性表达率呈逐渐增高趋势，HPV16E6与hWAPL在CINⅢ组和ICC组的阳性表达率与正常宫颈组、CINⅠ或Ⅱ组差异有统计学意义（P＜0．05）。CINⅢ组和ICC组HPV16E6蛋白阳性表达率差异有统计学意义（P＜0．05），而hWAPL蛋白的阳性表达率无明显差异（P＝0．372）。HPV16E6与hWAPL的表达强度在ICC组、CINⅢ组明显高于CINⅠ或Ⅱ组和正常宫颈组（P＜0．05），而ICC组与CINⅢ组之间无明显差异（P＞0．05）。64例HPV16E6阳性者中57例（89．06％）hWAPL蛋白表达阳性，62例阴性者中15例（24．19％）hWAPL蛋白表达阳性。经统计学处理，二者有明显相关性（P＜0．05），而且HPV16E6感染强度与hWAPL表达呈正相关（r＝0．595， P＜0．001）。结论 hWAPL基因是宫颈癌特异性高表达基因，具有癌基因特性，可能是宫颈癌发生机制中的重要环节，而且可能成为新的检测指标，用于宫颈鳞癌的基因诊断、治疗及明确病变程度。
목적：검측궁경불동급별병변조직중인유두류병독16 E6（HPV16 E6）급인반익기인（hWAPL）적표체，분석궁경암여HPV16 E6급hWAPL단백적상관성，위조기진단궁경암제공의거。방법채용면역조화방법검측궁경불동급별병변조직적석사포매괴，기중궁경상피세포내류변（CIN）Ⅰ혹CIN Ⅱ49례，CIN Ⅲ혹원위암33례，궁경침윤암（ICC）28례，정상궁경상피조직16례。결과 HPV16E6여hWAPL재정상궁경조、CINⅠ혹Ⅱ조、CINⅢ조화ICC조적조직중양성표체솔정축점증고추세，HPV16E6여hWAPL재CINⅢ조화ICC조적양성표체솔여정상궁경조、CINⅠ혹Ⅱ조차이유통계학의의（P＜0．05）。CINⅢ조화ICC조HPV16E6단백양성표체솔차이유통계학의의（P＜0．05），이hWAPL단백적양성표체솔무명현차이（P＝0．372）。HPV16E6여hWAPL적표체강도재ICC조、CINⅢ조명현고우CINⅠ혹Ⅱ조화정상궁경조（P＜0．05），이ICC조여CINⅢ조지간무명현차이（P＞0．05）。64례HPV16E6양성자중57례（89．06％）hWAPL단백표체양성，62례음성자중15례（24．19％）hWAPL단백표체양성。경통계학처리，이자유명현상관성（P＜0．05），이차HPV16E6감염강도여hWAPL표체정정상관（r＝0．595， P＜0．001）。결론 hWAPL기인시궁경암특이성고표체기인，구유암기인특성，가능시궁경암발생궤제중적중요배절，이차가능성위신적검측지표，용우궁경린암적기인진단、치료급명학병변정도。
Objective To investigate the expressions of human papillomavirus 1 6E6 (HPV1 6 E6 )and human wings apart-like gene (hWAPL)in different levels of cervical lesions and the relationship of HPV1 6E6 and hWAPL protein with cervical cancer,and to provide the reference for the early diagnosis of cervical cancer.Methods By immunohistochemistry,the paraffin-embedded blocks with different levels of cervical lesions were detected,including 49 patients with cervical intraepitelial neoplasia (CIN)Ⅰ or CIN Ⅱ,33 patients with CIN Ⅲ or carcinoma in situ,28 patients with invasive cervical carcinoma (ICC)and 1 6 patients with normal cervical epithelium.Results In normal, CIN Ⅰ or Ⅱ,CIN Ⅲ and ICC groups,the positive expression rates of HPV1 6E6 and hWAPL showed gradually increasing trend.The positive expression rates of HPV1 6E6 and hWAPL in CIN Ⅲ and ICC groups had statistical significance with those in normal and CIN Ⅰ or CIN Ⅱ groups (P<0.05 ).Between CIN Ⅲ and ICC groups,the positive expression rate of HPV1 6E6 had statistical significance (P<0.05),but the positive expression rate of hWAPL protein had no obvious difference (P=0.372).HPV1 6E6 and hWAPL expression strengthes in ICC group and CIN Ⅲgroup were obviously higher than those in CIN Ⅰ orⅡgroup and normal group (P<0.05),and there was no obvious difference between ICC and CIN Ⅲ groups (P>0.05 ).The 57 of 64 patients (89.06%)with HPV1 6E6 had the positive expression of hWAPL protein,1 5 of 62 patients (24.1 9%)with no HPV1 6E6 had the positive expression of hWAPL protein.By statistical analysis,both HPV1 6E6 and hWAPL protein had significant correlation (P<0.05),and it showed that the infection intensity of HPV1 6E6 and the expression of hWAPL had positive correlation (r=0.595,P<0.001 ).Conclusions The hWAPL may be a cervical cancer-specific gene and expresses highly,and it may be important in the pathogenesis of cervical cancer.The hWAPL may become the new target gene for the diagnosis, treatment and lesion identification of cervical cancer.