CAJ | 학술논문

烟酰胺腺嘌呤二核苷酸( NAD+/NADH)是目前已知300多种脱氢酶的辅酶，通过对NADH的检测，可以间接测定底物浓度或酶活力。如何利用电化学技术实现NADH的准确、快速、稳定检测，一直是电化学及生物传感领域的重要课题。碳纳米管( CNT)的发现为NADH的电化学检测注入新的生机。本文综述了近年来碳纳米管修饰电极在NADH电化学检测及脱氢酶生物传感器构建中的应用进展，并展望了其应用前景。
연선알선표령이핵감산( NAD+/NADH)시목전이지300다충탈경매적보매，통과대NADH적검측，가이간접측정저물농도혹매활력。여하이용전화학기술실현NADH적준학、쾌속、은정검측，일직시전화학급생물전감영역적중요과제。탄납미관( CNT)적발현위NADH적전화학검측주입신적생궤。본문종술료근년래탄납미관수식전겁재NADH전화학검측급탈경매생물전감기구건중적응용진전，병전망료기응용전경。
Nicotinamide adenine dinucleotide ( NADH ) is involved as a cofactor in over 300 enzymatic reaction of NAD+/NADH dependent dehydrogenases. The application of amperometric NADH sensors has evolved to provide a promising measurement technique for detection of substrate or enzymatic activity. However , the direct oxidation of NADH at ordinary electrodes often requires high overpotential and suffers from low sensitivity and the fouling of the electrode surface by its oxidation products. In recent years, carbon nanotubes ( CNT) are attracting growing attention in decreasing the high overpotential for NADH oxidation and minimizing the surface fouling. This paper introduces the research progress of the NADH electrochemical sensors based on CNT-modified electrodes, and foretells its application prospect.