CAJ | 학술논문

建立了血液中5种抗抑郁类药物的自动固相萃取-液相色谱-串联质谱( ASPE-LC-MS/MS)分析方法。样品经HLB固相萃取柱提取后,采用LC-MS/MS进行测定,外标法定量。在Waters AtlantisTM dC18反相柱上分离,梯度洗脱,流动相为甲醇-0.1%甲酸(1：4, V/V)溶液,质谱采集为电喷雾正离子多反应监测模式。采用基质匹配标准溶液校正,5种目标物在1~500μg/L范围内具有良好的线性关系,相关系数( R)大于0.9975,检出限在0.1~0.6μg/L之间；添加浓度水平为10,100和500μg/L时,方法回收率为70.6%~93.8%,相对标准偏差在3.9%~9.2%之间。本方法可用于血液中5种抗抑郁类药物的法庭与临床毒物分析。
건립료혈액중5충항억욱류약물적자동고상췌취-액상색보-천련질보( ASPE-LC-MS/MS)분석방법。양품경HLB고상췌취주제취후,채용LC-MS/MS진행측정,외표법정량。재Waters AtlantisTM dC18반상주상분리,제도세탈,류동상위갑순-0.1%갑산(1：4, V/V)용액,질보채집위전분무정리자다반응감측모식。채용기질필배표준용액교정,5충목표물재1~500μg/L범위내구유량호적선성관계,상관계수( R)대우0.9975,검출한재0.1~0.6μg/L지간；첨가농도수평위10,100화500μg/L시,방법회수솔위70.6%~93.8%,상대표준편차재3.9%~9.2%지간。본방법가용우혈액중5충항억욱류약물적법정여림상독물분석。
An efficient and accurate analytical method was developed for the simultaneous determination of 5 antidepressants in blood, by automated solid phase extraction-liquid chromatography tandem mass spectrometry ( ASPE-LC-MS/MS) . The samples were cleaned up by HLB solid phase extraction cartridge, and the analyses were carried out on LC-MS/MS. The chromatographic separation was performed on a Waters AtlantisTM dC18 column with gradient elution by using methanol and formic acid in water as mobile phases. Detection was carried out under the multiple reaction monitoring ( MRM) in positive ionization mode. Matrix-matched calibration curves with good correlation coefficients ( R>0 . 9975 ) were obtained in the concentration range of 1-500 μg/L. The detection limits of the method were 0. 1-0. 6 μg/L. The mean recoveries at the three spiked levels of 10-500 μg/L were 70 . 6%-93 . 8%, and the relative standard deviations were 3 . 9%-9. 2%. The method is applicable to analyze antidepressants in forensic and clinical toxicology.