CAJ | 학술논문

以琼脂糖珠作为固定 DNA 的载体,建立了可视化的快速检测尿液中 Hg2+的方法。 DNA 对 Hg2+特异性识别后,其构象发生变化,启动相邻序列DNA酶的类过氧化物酶催化活性,进而催化氧化双氧水介导的ABTS(2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸))体系,溶液显绿色。考察了各种物质用量对检测体系的影响。在最优实验条件下,体系在420 nm 处的吸光度与 Hg2+浓度呈良好线性关系,线性范围为5~200 nmol/L,检出限为2 nmol/L。应用于真实尿样中 Hg2+检测,加标回收率为95.1%~99.8%,相对标准偏差(n=5)为1.5%~3.1%。本方法对 Hg2+具有良好的选择性,且检测不受其它金属离子干扰。另外,以琼脂糖珠为固相载体,能实现高效快速分离,有效排除尿液中其它物质对显色的影响,提高了检测的准确度和灵敏度。
이경지당주작위고정 DNA 적재체,건립료가시화적쾌속검측뇨액중 Hg2+적방법。 DNA 대 Hg2+특이성식별후,기구상발생변화,계동상린서렬DNA매적류과양화물매최화활성,진이최화양화쌍양수개도적ABTS(2,2-련담-이(3-을기-분병새서-6-광산))체계,용액현록색。고찰료각충물질용량대검측체계적영향。재최우실험조건하,체계재420 nm 처적흡광도여 Hg2+농도정량호선성관계,선성범위위5~200 nmol/L,검출한위2 nmol/L。응용우진실뇨양중 Hg2+검측,가표회수솔위95.1%~99.8%,상대표준편차(n=5)위1.5%~3.1%。본방법대 Hg2+구유량호적선택성,차검측불수기타금속리자간우。령외,이경지당주위고상재체,능실현고효쾌속분리,유효배제뇨액중기타물질대현색적영향,제고료검측적준학도화령민도。
A novel colorimetric assay for the rapid detection of mercury ions in urine was established with agarose beads as carrier to capture DNA. Specific recognition of DNA towards mercury ions leads to conformational change and DNAzyme formation caused by DNA conformational change revealed peroxidase-like activities, catalyzing the H2 O2-mediated oxidation of 2, 2-azinobis ( 3-ethylbenzothiozoline )-6-sulfonic acid ( ABTS) to produce green color. The effects of the amount of various substances on detection system were investigated. Under optimal conditions, the absorbances at 420 nm of the system showed a good linear relationship with the concentration of mercury ions ranged from 5 to 200 nmol/L, the detection limit was 2 nmol/L. When tested Hg2+in real urine, the spiked recoveries were from 95. 1% to 99. 8% and the relative standard deviations ( n=5 ) were between 1 . 5% and 3 . 1%. This method had good selectivity for the detection of Hg2+avoiding interference from other metal ions. Furthermore, the fast and efficient separation was achieved with agarose beads as a solid phase carrier to effectively exclude the influence of other materials in urine on the color leading to the detection accuracy and high sensitivity.