CAJ | 학술논문

以喷涂了检测抗原黄曲霉毒素M1-BSA和驴抗鼠二抗形成检测线和质控线的硝酸纤维膜制备免疫层析试纸条,采用EDC/NHS法制备偶联了抗黄曲霉毒素M1单克隆抗体的免疫磁珠。免疫磁珠与待检样本混合,经捕获、磁分离后,浓缩重悬液直接用免疫层析试纸条检测,首次建立了集浓缩样本与免疫层析于一体的黄曲霉毒素M1快速检测法。该方法用于检测原料乳中黄曲霉毒素M1,检出限为0.1μg/L,低于我国制定的黄曲霉毒素M1限量标准(0.5μg/L),与其它真菌毒素和原料乳中常检违法添加物无交叉反应,分析结果与酶联免疫吸附法( ELISA)结果一致。本方法适合现场快速检测原料乳中黄曲霉毒素M1。
이분도료검측항원황곡매독소M1-BSA화려항서이항형성검측선화질공선적초산섬유막제비면역층석시지조,채용EDC/NHS법제비우련료항황곡매독소M1단극륭항체적면역자주。면역자주여대검양본혼합,경포획、자분리후,농축중현액직접용면역층석시지조검측,수차건립료집농축양본여면역층석우일체적황곡매독소M1쾌속검측법。해방법용우검측원료유중황곡매독소M1,검출한위0.1μg/L,저우아국제정적황곡매독소M1한량표준(0.5μg/L),여기타진균독소화원료유중상검위법첨가물무교차반응,분석결과여매련면역흡부법( ELISA)결과일치。본방법괄합현장쾌속검측원료유중황곡매독소M1。
Immunochromatographic strip was assembled with conjugate pad, sample pad, absorbent pad, and the nitrocellulose membrane on which aflatoxin M1-BSA and donkey anti-mouse antibody was sprayed and served as test line and control line, respectively. Anti-aflatoxin M1 coated immunomagnetic nanobeads was synthesized by an EDC/NHS method. Immunomagnetic nanobeads were mixed with sample for capturing and separation. The enrichment sample was detected by immunochromatographic strip. A rapid detection method of aflatoxin M1 containing sample enrichment and immunochromatographic assay was firstly set up. The limit of detection of this assay for aflatoxin M1 in raw milk was 0. 1 μg/L, which was lower than the legal limit of 0. 5 μg/L set by China. No cross-reactions were found with other mycotoxins and common illegal additives. The result of the method was in good agreement with that of ELISA. The assay can be applied for fast detection of aflatoxin M1 on-site in raw milk.