CAJ | 학술논문

本实验以巯基丙酸作为稳定剂,在水相条件下快速合成稳定性好的水溶性硒化锌量子点( ZnSe QDs),采用透射电子显微镜、X射线衍射、红外光谱、荧光以及紫外光谱法等对ZnSe QDs进行了材料表征。将得到的ZnSe QDs通过共价结合方式标记到牛分枝杆菌表面的MPB83蛋白抗体分子上,基于抗体与MPB83蛋白的特异性相互作用,建立了一种检测MPB83蛋白的光谱新方法。研究了pH值及温度对检测的影响,得到pH 8.5,37℃为适宜的体系酸度和温度。在优化的实验条件下,MPB83蛋白浓度在44~528 mg/L范围内,QDs的荧光强度与蛋白浓度间呈现良好的线性关系,此方法对MPB83蛋白的检出限为4.4 mg/L。该方法为实现准确而及时的诊断牛结核病提供一定理论依据。
본실험이구기병산작위은정제,재수상조건하쾌속합성은정성호적수용성서화자양자점( ZnSe QDs),채용투사전자현미경、X사선연사、홍외광보、형광이급자외광보법등대ZnSe QDs진행료재료표정。장득도적ZnSe QDs통과공개결합방식표기도우분지간균표면적MPB83단백항체분자상,기우항체여MPB83단백적특이성상호작용,건립료일충검측MPB83단백적광보신방법。연구료pH치급온도대검측적영향,득도pH 8.5,37℃위괄의적체계산도화온도。재우화적실험조건하,MPB83단백농도재44~528 mg/L범위내,QDs적형광강도여단백농도간정현량호적선성관계,차방법대MPB83단백적검출한위4.4 mg/L。해방법위실현준학이급시적진단우결핵병제공일정이론의거。
A basic water phase method was used for synthesizing water-soluble ZnSe quantum dots ( QDs ) with 3-mercaptopropionic acid ( MPA ) as ligands. The obtained MPA-ZnSe QDs were characterized by transmission electron microscopy, X-ray powder diffraction spectrometry, ultraviolet-visible spectrometry and fluorescence spectrometry. Then the prepared MPA-ZnSe QDs were conjugated with anti-MPB83 antibodies to form the fluorescent probe. As a result of specific interaction, MPB83 proteins were selectively captured by immunized MPA-ZnSe QDs which led to the change of a fluorescent signal. Based on this, a fluorescence-linked immunoassay method was demonstrated for the detection of MPB83 proteins. The influence of pH and temperature were studied, thus the optimum reaction condition ( pH 8. 5, 37 ℃) were obtained. Under optimal conditions, the quenched fluorescence intensity increased linearly with the concentration of MPB83 proteins ranging from 44 to 528 mg/L with the detection limit of 4. 4 mg/L. The approach provides theoretical basis for diagnosing bovine tuberculosis precisely and timely.