CAJ | 학술논문

目的：采用三酰甘油试剂盒检测冰冻解冻去甘油红细胞中甘油残留量，对该检测方法进行全面评价。方法对该方法的准确度、批内和批间精密度、线性范围及可报告范围等性能进行确认，以确保检测结果的准确、可靠。结果准确度：偏移小于或等于2．50％；批内精密度相对标准偏差（RSD ）≤1．0％，批间精密度RSD≤2．0％；在0．0078～2．0000 g/L浓度范围内，测定结果与标准溶液浓度呈线性相关，Y＝0．9752X＋0．0095（r2＝0．9998）；可报告范围下限为0．0078g/L，上限为64．0g/L。结论采用三酰甘油试剂盒检测甘油残留量的方法能满足采供血机构需求。
목적：채용삼선감유시제합검측빙동해동거감유홍세포중감유잔류량，대해검측방법진행전면평개。방법대해방법적준학도、비내화비간정밀도、선성범위급가보고범위등성능진행학인，이학보검측결과적준학、가고。결과준학도：편이소우혹등우2．50％；비내정밀도상대표준편차（RSD ）≤1．0％，비간정밀도RSD≤2．0％；재0．0078～2．0000 g/L농도범위내，측정결과여표준용액농도정선성상관，Y＝0．9752X＋0．0095（r2＝0．9998）；가보고범위하한위0．0078g/L，상한위64．0g/L。결론채용삼선감유시제합검측감유잔류량적방법능만족채공혈궤구수구。
Objective To evaluate the technology that analyzing the glycerol residual volume in the frozen and deglycerolized red blood cells with the triglyceride detection kits .Methods The important elements including operators ,instruments ,accuracy ,within-run precision ,between-run precision ,linear range and the reportable range were evaluated .Results The accuracy :Bias≤2 .50% , within-run precision RSD ≤1 .0% ,between-run precision RSD ≤2 .0% .A better linear relation was presented between the analysis results and the corresponding concentration of the reference materials in the concentration range from 0 .007 8 g/L to 2 .000 0 g/L , Y=0 .975 2X+0 .009 5(r2 =0 .999 8) .The reportable lower limit was 0 .007 8 g/L and the upper limit was 64 .0 g/L .Conclusion The technology that analyzing the glycerol residual volume with the triglyceride detection kits is satisfied demond for the blood bank .