国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
8期
1036-1038
,共3页
抗核抗体%间接免疫荧光法%蛋白质印迹法
抗覈抗體%間接免疫熒光法%蛋白質印跡法
항핵항체%간접면역형광법%단백질인적법
antinuclear antibody%indirect immunofluorescence assay%Western blotting
目的：探讨间接免疫荧光法检测抗核抗体（ANA）结果与蛋白质印迹法检测ANA结果之间的相关性，以及两种方法联合检测在自身免疫性疾病中的诊断价值。方法收集2011年4月至2013年4月同时用间接免疫荧光法与蛋白质印迹法检测ANA的患者资料，并对检测结果进行对比分析。结果间接免疫荧光法检出ANA阳性率明显低于蛋白质印迹法（χ2＝158．69， P＜0．01）。间接免疫荧光法检测ANA阳性标本中，有20例蛋白质印迹法检测为阴性；蛋白质印迹法检测ANA为阳性的775例标本中，有295例（38．06％）间接免疫荧光法检测为阴性。间接免疫荧光法检测A N A的荧光模型与特异性A N A之间没有绝对的规律。结论蛋白质印迹法检测ANA的敏感性较高，间接免疫荧光存在假阴性的可能，选择一个敏感性较高的检测组合十分必要。
目的：探討間接免疫熒光法檢測抗覈抗體（ANA）結果與蛋白質印跡法檢測ANA結果之間的相關性，以及兩種方法聯閤檢測在自身免疫性疾病中的診斷價值。方法收集2011年4月至2013年4月同時用間接免疫熒光法與蛋白質印跡法檢測ANA的患者資料，併對檢測結果進行對比分析。結果間接免疫熒光法檢齣ANA暘性率明顯低于蛋白質印跡法（χ2＝158．69， P＜0．01）。間接免疫熒光法檢測ANA暘性標本中，有20例蛋白質印跡法檢測為陰性；蛋白質印跡法檢測ANA為暘性的775例標本中，有295例（38．06％）間接免疫熒光法檢測為陰性。間接免疫熒光法檢測A N A的熒光模型與特異性A N A之間沒有絕對的規律。結論蛋白質印跡法檢測ANA的敏感性較高，間接免疫熒光存在假陰性的可能，選擇一箇敏感性較高的檢測組閤十分必要。
목적：탐토간접면역형광법검측항핵항체（ANA）결과여단백질인적법검측ANA결과지간적상관성，이급량충방법연합검측재자신면역성질병중적진단개치。방법수집2011년4월지2013년4월동시용간접면역형광법여단백질인적법검측ANA적환자자료，병대검측결과진행대비분석。결과간접면역형광법검출ANA양성솔명현저우단백질인적법（χ2＝158．69， P＜0．01）。간접면역형광법검측ANA양성표본중，유20례단백질인적법검측위음성；단백질인적법검측ANA위양성적775례표본중，유295례（38．06％）간접면역형광법검측위음성。간접면역형광법검측A N A적형광모형여특이성A N A지간몰유절대적규률。결론단백질인적법검측ANA적민감성교고，간접면역형광존재가음성적가능，선택일개민감성교고적검측조합십분필요。
Objective To investigate the correlation between indirect immunofluorescence (IFT ) method and Western blot assay for the diagnosis of antinuclear antibody (ANA) ,based on which to evaluate the diagnostic value of IFT combined with Western blot for the autoimmune disease .Methods Medical materials from patients received IFT combined with Western blot for the diagnosis of autoimmune disease from April 2011 to April 2013 were included in this study .On this basis ,a comparative study was performed to evaluate the diagnosis results .Results The positive rates obtained using IFT method were significantly lower than that of West-ern blot assay(χ2 =158 .69 ,P<0 .01) .Among the samples with positive results using IFT method ,20 samples were considered as negative using Western blot assay .On the contrary ,among the 775 samples with positive results using Western blot assay ,295 sam-ples(38 .06% ) were considered to be negative using IFT method .No absolute association was established between the fluorescent pattern and specificity of the IFT method in the diagnosis of ANA .Conclusion The sensitivity of Western blot assay for the diag-nosis of ANA was comparatively higher than that of IFT method .A possibility of false negative was noted in the IFT method .It is necessary to select a combined method with higher sensitivity for the diagnosis of ANA .
