国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
8期
1029-1031
,共3页
童明华%刘琼%王敏%梁日初%胡敏%郑荣%徐霞%董政%丁海榕%彭凤英
童明華%劉瓊%王敏%樑日初%鬍敏%鄭榮%徐霞%董政%丁海榕%彭鳳英
동명화%류경%왕민%량일초%호민%정영%서하%동정%정해용%팽봉영
耐甲氧西林葡萄球菌%甲氧西林敏感葡萄球菌%营养条件改变%mecA基因%耐药机制
耐甲氧西林葡萄毬菌%甲氧西林敏感葡萄毬菌%營養條件改變%mecA基因%耐藥機製
내갑양서림포도구균%갑양서림민감포도구균%영양조건개변%mecA기인%내약궤제
methicillin-resistant Staphylococcus%methicillin-susceptible Staphylococcus%nutritional conditions change%mecA gene%drug resistance mechanism
目的：改变耐甲氧西林葡萄球菌（M RS ）的营养条件，连续传代诱导，使M RS诱变为甲氧西林敏感葡萄球菌（M SS ）。方法将临床分离并经确定为M RS的菌株接种于不同营养条件环境中进行连续传代培养，定期做药敏试验，观察M RS的表型变化及生化反应有无改变。同时采用PCR法检测原菌株及通过传代诱导其脱耐诱变菌株 mecA 基因的携带情况并测序比对。结果53株M RS经过不同次传代，有6株菌脱耐诱变为M SS。这6株菌在未进行诱导前检测均含有 mecA基因，诱导后有2株菌未检测到 mecA基因，其他4株菌 mecA基因条带显示极弱。结论临床分离的M RS能通过不同营养条件的传代培养诱变脱耐转为M SS ，M RS的 mecA基因能随生长环境改变丢失或表达下降，M RS与其脱耐菌株在基因组学上存在差异。
目的：改變耐甲氧西林葡萄毬菌（M RS ）的營養條件，連續傳代誘導，使M RS誘變為甲氧西林敏感葡萄毬菌（M SS ）。方法將臨床分離併經確定為M RS的菌株接種于不同營養條件環境中進行連續傳代培養，定期做藥敏試驗，觀察M RS的錶型變化及生化反應有無改變。同時採用PCR法檢測原菌株及通過傳代誘導其脫耐誘變菌株 mecA 基因的攜帶情況併測序比對。結果53株M RS經過不同次傳代，有6株菌脫耐誘變為M SS。這6株菌在未進行誘導前檢測均含有 mecA基因，誘導後有2株菌未檢測到 mecA基因，其他4株菌 mecA基因條帶顯示極弱。結論臨床分離的M RS能通過不同營養條件的傳代培養誘變脫耐轉為M SS ，M RS的 mecA基因能隨生長環境改變丟失或錶達下降，M RS與其脫耐菌株在基因組學上存在差異。
목적：개변내갑양서림포도구균（M RS ）적영양조건，련속전대유도，사M RS유변위갑양서림민감포도구균（M SS ）。방법장림상분리병경학정위M RS적균주접충우불동영양조건배경중진행련속전대배양，정기주약민시험，관찰M RS적표형변화급생화반응유무개변。동시채용PCR법검측원균주급통과전대유도기탈내유변균주 mecA 기인적휴대정황병측서비대。결과53주M RS경과불동차전대，유6주균탈내유변위M SS。저6주균재미진행유도전검측균함유 mecA기인，유도후유2주균미검측도 mecA기인，기타4주균 mecA기인조대현시겁약。결론림상분리적M RS능통과불동영양조건적전대배양유변탈내전위M SS ，M RS적 mecA기인능수생장배경개변주실혹표체하강，M RS여기탈내균주재기인조학상존재차이。
Objective To reversing methicillin-resistant Staphylococcus(MRS) to methicillin-susceptible Staphylococcus(MSS) by changing nutritional conditions and continuous transfer of culture .Methods MRS trains separating from clinical specimens were cultured in different conditions ,continuous cultural transfer ,and drug sensitive test were proceeded periodically to observe the phe-notypic and chemical reaction change of MRS .The mecA gene were detected of the original and mutant strains by polymerase chain reaction(PCR) ,then the gene sequenced and compared .Results 53 MRS strains were studied .6 strains were phenotype successful-ly converted to MSS in different cultural conditions ,among them mecA gene was undetected in 2 strains ,and down expressed in 4 strains .Conclusion The MRS strains separated from clinical specimens may revert to MSS by culture under different nutritional conditions .The mecA gene of MRS may be lost or lower expressed and the MRS and mutant strains may be different in genomics .
