CAJ | 학술논문

目的：建立血清中草甘膦定性、定量测定的紫外分光光度法，为临床草甘膦中毒提供诊疗依据。方法取0．5mL血清加10％高氯酸甲醇溶液0．2mL充分振荡，以10000r/min高速离心5min，取上清液进行亚硝基化反应，血清空白做基线，患者血清亚硝基化液50μL紫外扫描。结果血清中草甘膦最大吸收峰（243±l）nm，浓度在10．0～60．0μg/mL范围内呈线性，回归方程为Y＝0．01738X＋0．0363（r＝0．9998），回收率为85．5％～102．4％，相对标准差（RSD）为3．50％～4．90％。日内、日间RSD分别为3．79％～5．10％和3．88％～4．55％，最低检出浓度5．0μg/mL。结论该方法操作简便、分析快速、结果准确。为临床诊断草甘膦中毒提供了一个简便准确的检测方法。
목적：건립혈청중초감련정성、정량측정적자외분광광도법，위림상초감련중독제공진료의거。방법취0．5mL혈청가10％고록산갑순용액0．2mL충분진탕，이10000r/min고속리심5min，취상청액진행아초기화반응，혈청공백주기선，환자혈청아초기화액50μL자외소묘。결과혈청중초감련최대흡수봉（243±l）nm，농도재10．0～60．0μg/mL범위내정선성，회귀방정위Y＝0．01738X＋0．0363（r＝0．9998），회수솔위85．5％～102．4％，상대표준차（RSD）위3．50％～4．90％。일내、일간RSD분별위3．79％～5．10％화3．88％～4．55％，최저검출농도5．0μg/mL。결론해방법조작간편、분석쾌속、결과준학。위림상진단초감련중독제공료일개간편준학적검측방법。
Objective To establish a qualitative and quantitative determination of glyphosate in serum using ultraviolet spectro-photometry(UV) to provide basis for clinical diagnosing and treating glyphosate poisoning .Methods The mixture of 0 .5 mL serum and 0 .2 mL 10% methanol solution of perchloric acid was shocked and centrifuged with 10 000 r/min for 5 min .A nitrosyla-tion reaction conducted on supernatant and 50 μL serum nitrosylation liquid was detected by UV scanning .Results The results of serum theophylline absorption maxima was(243 ± l) nm and the concentration of 10 .0-60 .0 μg/mL range linear regression equa-tion was Y=0 .0173 8X+0 .036 3(r= 0 .999 8) .The recovery rate was from 85 .5% to 102 .4% and the relative standard deviation (RSD) was from 3 .50% to 4 .90% .The intra-day and inter-day RSD were 3 .79% -5 .10% and 3 .88% -4 .55% .The minimum de-tectable concentration was 5μg/mL .Conclusion This method is simple ,rapid and accurate results for detecting glyphosate poison-ing .