CAJ | 학술논문

目的：研究单核细胞趋化蛋白-1（MCP-1）在慢性乙型病毒性肝炎（CHB）患者肝脏活检组织和血清的表达。方法采用酶联免疫吸附试验（ELISA）对124例CHB炎患者和20例对照组血清MCP-1水平进行检测，采用免疫组化和实时荧光定量PCR检测CHB患者肝组织中MCP-1蛋白和mRNA表达。结果对照组和CHB组血清中MCP-1水平分别是（265±44）ng/L和（157±80）ng/L ，CHB组血清MCP-1水平显著低于对照组（t＝5．355，P＜0．01）。CHB患者血清MCP-1水平与肝组织炎症分级（r＝0．353，P＝0．011）和纤维化分期（r＝0．496，P＜0．01）呈正相关。肝组织中MCP-1 mRNA水平与炎症分级和纤维化分期呈正相关（ r＝0．465，P＜0．01；r＝0．493，P＜0．01）。肝组织中M C P-1蛋白表达与炎症分级和纤维化分期呈正相关（ r＝0．486， P＝0．001；r＝0．542，P＜0．01）。结论 CHB患者血清中MCP-1水平下调，MCP-1表达水平与肝组织炎症分级、纤维化分期呈正相关。
목적：연구단핵세포추화단백-1（MCP-1）재만성을형병독성간염（CHB）환자간장활검조직화혈청적표체。방법채용매련면역흡부시험（ELISA）대124례CHB염환자화20례대조조혈청MCP-1수평진행검측，채용면역조화화실시형광정량PCR검측CHB환자간조직중MCP-1단백화mRNA표체。결과대조조화CHB조혈청중MCP-1수평분별시（265±44）ng/L화（157±80）ng/L ，CHB조혈청MCP-1수평현저저우대조조（t＝5．355，P＜0．01）。CHB환자혈청MCP-1수평여간조직염증분급（r＝0．353，P＝0．011）화섬유화분기（r＝0．496，P＜0．01）정정상관。간조직중MCP-1 mRNA수평여염증분급화섬유화분기정정상관（ r＝0．465，P＜0．01；r＝0．493，P＜0．01）。간조직중M C P-1단백표체여염증분급화섬유화분기정정상관（ r＝0．486， P＝0．001；r＝0．542，P＜0．01）。결론 CHB환자혈청중MCP-1수평하조，MCP-1표체수평여간조직염증분급、섬유화분기정정상관。
Objective To investigate the expression of monocyte chemoattractant protein -1(MCP-1) in serum and liver biopsies of patients with chronic hepatitis B (CHB) .Methods 124 patients with CHB and 20 normal controls were explored .MCP-1 was de-tected by ELISA ,real time PCR and immunologic histochemistry ,respectively .The concentrations of MCP-1 in serum were meas-ured by ELISA .Real-time PCR and immunohistochemistry were applied to detect mRNA and protein expression of MCP-1 in the liver biopsy ,respectively .Results The concentrations of MCP-1 were (157 ± 80)ng/L in serum of CHB patients and (265 ± 44) ng/L in control groups .There were significantly different between the two groups (t=5 .355 ,P<0 .01) .There was positive correla-tions between MCP-1 concentration in serum and grading of inflammation(r=0 .353 ,P=0 .011) ,staging of fibrosis(r=0 .496 ,P<0 .01) in CHB patients .And positive correlations were also found between mRNA levels of MCP-1 and grading of inflammation(r=0 .465 ,P<0 .01) ,staging of fibrosis(r=0 .493 ,P<0 .01) in liver biopsies of patients with CHB .At the same time positive correla-tions was observed between immunohistochemistry score and grading of inflammation (r=0 .486 ,P=0 .001) ,staging of fibrosis(r=0 .542 ,P<0 .01) .Conclusion Concentrations of MCP-1 are decreased in the sera of CHB patients .Positive correlation lies between the expression of MCP-1 and histological grading and staging of CHB .