CAJ | 학술논문

目的：了解执行长期海上航行官兵任务前、中、后期口腔颊黏膜细菌分布变化，为舰艇官兵长期海上航行期间口腔疾病的防治提供依据。方法用采样拭子采集口腔颊黏膜标本164份，进行细菌培养，根据细菌在不同培养基上的生长情况进行细菌分纯，取纯培养细菌进行手工鉴定及基质辅助激光解吸电离飞行时间质谱仪鉴定。结果从164份口腔颊黏膜标本分离出病原菌18种64株。任务后期与前期比较，病原菌分离数量增加，栖居菌分离数量减少。医疗组与机电组比较，在任务中、后期病原菌检出率之间差异有统计学意义（P＜0．05），栖居菌检出率之间差异无统计学意义（P＞0．05）。结论长时间海上航行，舰员易发生航行疲劳，导致机体免疫功能下降，口腔菌群失调，正常栖居菌数减少，而病原菌数增加，容易引发口腔及其他系统疾病。
목적：료해집행장기해상항행관병임무전、중、후기구강협점막세균분포변화，위함정관병장기해상항행기간구강질병적방치제공의거。방법용채양식자채집구강협점막표본164빈，진행세균배양，근거세균재불동배양기상적생장정황진행세균분순，취순배양세균진행수공감정급기질보조격광해흡전리비행시간질보의감정。결과종164빈구강협점막표본분리출병원균18충64주。임무후기여전기비교，병원균분리수량증가，서거균분리수량감소。의료조여궤전조비교，재임무중、후기병원균검출솔지간차이유통계학의의（P＜0．05），서거균검출솔지간차이무통계학의의（P＞0．05）。결론장시간해상항행，함원역발생항행피로，도치궤체면역공능하강，구강균군실조，정상서거균수감소，이병원균수증가，용역인발구강급기타계통질병。
Objective To study the bacteriological change in buccal mucosa of sailors during long-term voyage and provide basis for the prevention and treatment of buccal diseases .Methods Bacterial cultivation ,isolation of 164 buccal mucosa specimens were used and analyzed by both handwork and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) identification .Results 18 species and 64 strains of pathogenic bacteria were isolated from the whole samples .The num-ber of pathogenic bacteria increased while the number of dwelling bacteria decreased significantly later in the voyage .Comparing with the medical group ,the number of pathogenic bacteria detected in mechanical and electrical group was significantly high (P<0 .05) ,during and after the long-term voyage .As for dwelling bacteria ,no significant difference was observed in between the two groups(P>0 .05) .Conclusion Sailors to fatigue during long-term voyage indicates by decline of immunological function and buccal dysbacteriosis ,which easily lead to buccal and other system diseases .