CAJ | 학술논문

背景：人工关节周围产生骨溶解是关节松动失败的重要原因，各国学者都在寻求一种能有效抑制骨溶解反应的药物，以减少假体松动的发生。 　　目的：观察不同剂量淫羊藿苷干预人工关节磨损微粒诱导骨溶解的效果。 　　方法：将钛合金及骨水泥磨损微粒制成的混悬液分别置入清洁小鼠颅盖骨进行骨溶解建模后，空白组滴入生理盐水到颅盖骨中，药物干预组给予不同浓度的淫羊藿苷(剂量分别为30，60，120 mg/kg)灌胃，每日1次。建模2周后处死小鼠，在显微镜下观察小鼠颅盖骨的结构。经苏木精-伊红染色和免疫组化分析计算破骨细胞数量和小鼠颅盖骨溶解面积的变化。 　　结果与结论：与空白组比较，钛合金微粒和骨水泥微粒建模后的小鼠颅盖骨切片上的骨溶解陷窝数量及破骨细胞数明显增加，图像分析骨溶解陷窝面积也增大(P <0.05)。淫羊藿苷干预后骨溶解面积减小及破骨细胞数量减少(P<0.05)，以120 mg/kg灌胃组最为显著，其次是60 mg/kg，30 mg/kg。结果可见钛磨损微粒和骨水泥磨损微粒能促进破骨细胞的增殖，诱导骨溶解；淫羊藿苷能抑制磨损微粒诱导的破骨细胞形成，从而抑制骨溶解。
배경：인공관절주위산생골용해시관절송동실패적중요원인，각국학자도재심구일충능유효억제골용해반응적약물，이감소가체송동적발생。 　　목적：관찰불동제량음양곽감간예인공관절마손미립유도골용해적효과。 　　방법：장태합금급골수니마손미립제성적혼현액분별치입청길소서로개골진행골용해건모후，공백조적입생리염수도로개골중，약물간예조급여불동농도적음양곽감(제량분별위30，60，120 mg/kg)관위，매일1차。건모2주후처사소서，재현미경하관찰소서로개골적결구。경소목정-이홍염색화면역조화분석계산파골세포수량화소서로개골용해면적적변화。 　　결과여결론：여공백조비교，태합금미립화골수니미립건모후적소서로개골절편상적골용해함와수량급파골세포수명현증가，도상분석골용해함와면적야증대(P <0.05)。음양곽감간예후골용해면적감소급파골세포수량감소(P<0.05)，이120 mg/kg관위조최위현저，기차시60 mg/kg，30 mg/kg。결과가견태마손미립화골수니마손미립능촉진파골세포적증식，유도골용해；음양곽감능억제마손미립유도적파골세포형성，종이억제골용해。
BACKGROUND:Osteolysis surrounding artificial joints is the main reason for the failure of joint loosening. Scholars al over the world are seeking an effective drug to inhibit osteolysis so as to reduce the occurrence of prosthetic loosening. OBJECTIVE:To observe the effects of different doses of icari n on osteolysis induced by wear particles. METHODS:The wear debris (Ti al oy and bone cement) was put into mouse cranium to create mouse models of calvarial osteolysis. Physiological saline was dropped in the cranium in the blank group. Different concentrations of icari n (30, 60 and 120 mg/kg) were given in the drug intervention group, by intragastric administration, once a day. At 2 weeks after model induction, mice were sacrificed. The structure of mouse cranium was observed under a microscope. Hematoxylin-eosin staining and immunohistochemical staining were performed. The number of osteoclasts and the changes in the calvarial osteolysis area of mice after intervention were calculated. RESULTS AND CONCLUSION:Compared with the blank group, the number of absorption pits of bone slices that were stimulated by wear debris (Ti al oy and bone cement) and the number of osteoclasts were significantly increased, and absorption pits area was also bigger (P<0.05). After intervention with icari n, osteolysis area and the number of osteoclasts became less (P<0.05), especial y in the 120 mg/kg gavage group, fol owed by 60 mg/kg and 30 mg/kg. Results suggested that Ti particles and bone cement particles can promote osteoclast proliferation and osteolysis. Icari n can suppress the formation of osteoclasts induced by particles, resulting in inhibiting osteolysis.