CAJ | 학술논문

以蓝靛果忍冬优良品系L1-8为试验材料，取其休眠枝条萌发的腋芽为外植体，将培养基（MS、White、WPM、A3）、激素IBA（0.1、0.2、0.3、0.4 mg·L-1）、6-BA（1.0、1.5、2.0、2.5 mg·L-1）和GA3（0.5、1.0、1.5、2.0 mg·L-1）等处理组合进行4因素、4水平的正交试验，以优化筛选最佳分化培养基，为完善蓝靛果忍冬组培快繁技术提供技术支撑。结果表明，筛选出最优分化培养基为MS+6-BA 2.0 mg·L-1+IBA 0.3 mg·L-1+GA31.5 mg·L-1（分化率可达95.92%）；基本培养基和不同激素对分化率产生影响，在4个因素中，基本培养基和6-BA是影响分化率的主要因素，IBA和GA3为次要因素；筛选出的不同培养基组合对增殖系数影响不同，最高为4.14，且芽苗生长状况良好；继代周期为30 d时，增殖效果最好。
이람전과인동우량품계L1-8위시험재료，취기휴면지조맹발적액아위외식체，장배양기（MS、White、WPM、A3）、격소IBA（0.1、0.2、0.3、0.4 mg·L-1）、6-BA（1.0、1.5、2.0、2.5 mg·L-1）화GA3（0.5、1.0、1.5、2.0 mg·L-1）등처리조합진행4인소、4수평적정교시험，이우화사선최가분화배양기，위완선람전과인동조배쾌번기술제공기술지탱。결과표명，사선출최우분화배양기위MS+6-BA 2.0 mg·L-1+IBA 0.3 mg·L-1+GA31.5 mg·L-1（분화솔가체95.92%）；기본배양기화불동격소대분화솔산생영향，재4개인소중，기본배양기화6-BA시영향분화솔적주요인소，IBA화GA3위차요인소；사선출적불동배양기조합대증식계수영향불동，최고위4.14，차아묘생장상황량호；계대주기위30 d시，증식효과최호。
Lonicera edulis strain, designated strain L1-8 that provided by Chinese Academy of Science, Northeast Institute of Geography and Agricultural Ecology Institute , was applied as material in this research. The dormant branches of axil ary buds were used as explants for germination. The medium (MS, White, WPM, A3), hormone IBA (0.1, 0.2, 0.3, 0.4 mg·L-1), 6-BA (1.0, 1.5, 2.0, 2.5 mg·L-1) and GA3 (0.5, 1.0, 1.5, 2.0 mg·L-1) were applied for the four factors-four levels orthogonal experiment which in order to optimize the best differentiation medium and provide technical support for the perfection of tissue culture technology of Lonicera edulis micropropagation. The results showed that MS medium containing 2.0 mg·L-1 6-BA , 0.3 mg·L-1 IBA and 1.5 mg·L-1 GA3(The differentiation rate was 95.92%)was screened as the optimum differentiation medium. The basic medium and the different hormone both could affect the differentiation rate. In the four factors, the basic medium and 6-BA were the main factors affected the differentiation rate, whereas IBA and GA3 were the secondary factors. Screened on different culture combinations had different effects on multiplication. The multiplication was as high as 4.14 with the best growth. Subculture cycle was 30 d and the effect on multiplication was the best.