CAJ | 학술논문

以美国红枫‘白兰地’（Acer rubrum‘Brandywine’）的带腋芽的茎段为外植体，进行了组织培养与快繁技术研究，结果表明：1）外植体的最佳取材时段为3-5月，最佳消毒方案是用3％的 NaClO 处理8 min ，污染率仅为2.22％，存活率达91.11％．2）启动培养中，3因素对其影响的主次顺序依次为培养基，IBA ，6-BA ．在WPM ，MS ，1/2MS ，DKW 4种培养基中，WPM 对外植体启动培养影响显著高于其他3种，最佳培养基配方为WPM ＋ IBA 0.5 mg/L＋6-BA 2.0 mg/L ．3）增殖培养的最佳培养基配方为WPM＋0.002 mg/L TDZ＋0.4 mg/L NAA ，增殖系数为1.37．4）壮苗培养的最佳培养基配方为WPM＋0.5 mg/L NAA ＋1.0 mg/L GA3，苗的长势最好，茎粗且伸长明显，叶大且绿．5）根诱导培养中，4因素对其影响的主次顺序依次为6-BA ，GA3，培养基，IBA ，最佳培养基配方为MS＋2.0 mg/L IBA＋1.0 mg/L GA3，生根率高达93.3％．6）组培苗的炼苗移栽以先闭盖锻炼6 d ，后开盖锻炼2 d ，再移栽到沙与腐殖质等比混合的基质中，存活率最高，达86.7％．
이미국홍풍‘백란지’（Acer rubrum‘Brandywine’）적대액아적경단위외식체，진행료조직배양여쾌번기술연구，결과표명：1）외식체적최가취재시단위3-5월，최가소독방안시용3％적 NaClO 처리8 min ，오염솔부위2.22％，존활솔체91.11％．2）계동배양중，3인소대기영향적주차순서의차위배양기，IBA ，6-BA ．재WPM ，MS ，1/2MS ，DKW 4충배양기중，WPM 대외식체계동배양영향현저고우기타3충，최가배양기배방위WPM ＋ IBA 0.5 mg/L＋6-BA 2.0 mg/L ．3）증식배양적최가배양기배방위WPM＋0.002 mg/L TDZ＋0.4 mg/L NAA ，증식계수위1.37．4）장묘배양적최가배양기배방위WPM＋0.5 mg/L NAA ＋1.0 mg/L GA3，묘적장세최호，경조차신장명현，협대차록．5）근유도배양중，4인소대기영향적주차순서의차위6-BA ，GA3，배양기，IBA ，최가배양기배방위MS＋2.0 mg/L IBA＋1.0 mg/L GA3，생근솔고체93.3％．6）조배묘적련묘이재이선폐개단련6 d ，후개개단련2 d ，재이재도사여부식질등비혼합적기질중，존활솔최고，체86.7％．
With stems of axillary buds explants of A cer rubrum Brandy ,the tissue culture and rapid propa-gation procedure were studied .The results showed that (1) the best time to draw the material from the brandy was March to May ,and the optimal disinfection of explants was processed 8 minutes with 3% Na-ClO ,and the pollution rate was only 2 .22% ,the survival rate was 91 .11% .(2) The sequence of influence on Axillary bud initiation culture was as follows :medium ,IBA ,6-BA ,and the effect of WPM medium on primary culture was better than others ,the optimal medium was WPM +0 .5 mg/L IBA+2 .0 mg/L 6-BA . (3) The optimal medium of axillary bud was WPM +0 .001 mg/L TDZ+0 .4 mg/L NAA ,and the propaga-tion ratio was 1 .37 .(4) WPM+0 .5 mg/L NAA +1 .0 mg/L GA3 was the optimal medium to tube plant-let growing ,stem length were higher and stronger ,leaves were bigger and greener apparently than others under this condition .(5) The sequence of influence on root induction culture is as follows :6-BA ,GA3 ,me-dium ,IBA ,and the optimal medium is MS + 2 .0 mg/L IBA + 1 .0 mg/L GA3 ,and rooting rate reaches 93 .3% .(6) After 6 days’ closed cap and then 2 days’ opened cap to practice seedlings ,surviving of tube plantlet rates could reach 86 .7% w hen transplant into substrate w hich the ratio of sand and humus con-tents was 1∶1 .