CAJ | 학술논문

目的：评价 PREVL Isola 全自动微生物标本接种仪对常见标本的接种效果。方法用自动接种仪接种痰液标本与尿液标本各200份、咽拭子标本100份、支气管吸出物100份、血培养阳性标本100份（仪器法），同时用传统手工接种法（手工法）接种同样的标本，培养24～48 h 后观察菌落生长情况，比较接种仪接种与手工接种效果的差异。结果手工法和仪器法对700份标本培养24 h 后菌落生长阳性率分别为34．7％、40．4％，仪器法阳性率高于手工法（χ2＝6．69，P ＜0．05）。仪器法培养阳性的痰液标本中有19份经鉴定认为有意义的菌株而手工法未培养出；仪器接种法培养24 h 后单个菌落较多，多数可直接上机鉴定，报告时间较手工法缩短24 h。尿培养阳性标本中有10份仪器法较手工法菌落计数高1个数量级。血培养阳性标本经接种仪初次接种培养阳性率为93％，而手工法为82％。咽拭子标本及支气管吸出物培养显示，仪器接种法除阳性率高于手工法外，单个菌落的分离效果亦优于手工法。结论PREVL Isola 全自动微生物标本接种仪可以提高培养的阳性率，优化单个菌落分离效果，缩短了检出时间，并可确保操作人员的安全。
목적：평개 PREVL Isola 전자동미생물표본접충의대상견표본적접충효과。방법용자동접충의접충담액표본여뇨액표본각200빈、인식자표본100빈、지기관흡출물100빈、혈배양양성표본100빈（의기법），동시용전통수공접충법（수공법）접충동양적표본，배양24～48 h 후관찰균락생장정황，비교접충의접충여수공접충효과적차이。결과수공법화의기법대700빈표본배양24 h 후균락생장양성솔분별위34．7％、40．4％，의기법양성솔고우수공법（χ2＝6．69，P ＜0．05）。의기법배양양성적담액표본중유19빈경감정인위유의의적균주이수공법미배양출；의기접충법배양24 h 후단개균락교다，다수가직접상궤감정，보고시간교수공법축단24 h。뇨배양양성표본중유10빈의기법교수공법균락계수고1개수량급。혈배양양성표본경접충의초차접충배양양성솔위93％，이수공법위82％。인식자표본급지기관흡출물배양현시，의기접충법제양성솔고우수공법외，단개균락적분리효과역우우수공법。결론PREVL Isola 전자동미생물표본접충의가이제고배양적양성솔，우화단개균락분리효과，축단료검출시간，병가학보조작인원적안전。
Objective To evaluate the inoculation effect of PREVL Isola automatic inoculation system for common clinical specimens.Methods 200 sputum samples,200 urine specimens,100 throat swabs,100 bronchial aspirates and 100 blood culture positive specimens were inoculated by automatic inoculating appa-ratus.at the same the same specimens above were inoculated by traditional manual method.The growth of colonies was observed after 24 h to 48h of culture.The differences between inoculation effect were com-pared between inoculation system and manual method.Results After 24h culture the positive rate of 700 specimens was 34.7% (by manual method)and 40.4% (by instruments)respectively.The positive rate of instrumental method was higher than that of manual method (χ2 =6.69,P <0.05).19 significant strains from sputum samples inoculated by instrument were not detected by manual inoculation method.There were more single colonies after 24 h culture inoculated by Instrument method,and most of them can be i-dentified directly by machine,thus the report time could be shortened 24 h compared with manual method. The bacterial count of 10 strains inoculated by Instrument method was an order of magnitude higher com-pared with manual method among urine culture positive samples.The first subcultivation positive rate of instrumental inoculation method among positive blood culture samples was 93% compared to 82% for manual method.Throat swab specimens and bronchial aspirate culture showed that the separation effect of single colony inoculated by Instrument method was superior to the manual method besides the positive rate was higher than the manual method.Conclusion PREVL Isola automatic minoculation system can improve the positive rate of culture and optimize the single colony isolation.Meanwhile it can shorten the detection period and ensure the safety of operating personnel.