CAJ | 학술논문

目的：探讨没食子鞣质及其有效提取物对口腔细菌生物膜中葡萄糖转移酶（GTF）活性的影响及其防龋的作用。方法采用硫酸铵沉淀法提取粗酶，Neson-Somogyi 法测定还原糖，G250微量蛋白定量 GTF 还原糖计算 GTF 活性单位，实验分为阴性对照组（空白组），阳性对照组（为没有干预处理形成的细菌生物膜），没食子鞣质组和有效提取物（没食子酸＋没食子酸甲酯）组。评价实验药物没食子鞣质和有效提取物对口腔细菌生物膜中GTF 活性的影响。结果经没食子鞣质及其有效提取物作用后，没食子鞣质组24 h 的口腔细菌生物膜中 GTF 活性为（0．0186±0．077），有效提取物组 GTF 活性为（0．0527±0．035），GTF 的活性受到明显抑制，与对照组 GTF 活性（0．6601±0．204）相比差异均有统计学意义（P ＜0．05）。结论实验药物没食子鞣质及其有效提取物可能通过抑制细菌生物膜中 GTF 活性而发挥防龋作用。
목적：탐토몰식자유질급기유효제취물대구강세균생물막중포도당전이매（GTF）활성적영향급기방우적작용。방법채용류산안침정법제취조매，Neson-Somogyi 법측정환원당，G250미량단백정량 GTF 환원당계산 GTF 활성단위，실험분위음성대조조（공백조），양성대조조（위몰유간예처리형성적세균생물막），몰식자유질조화유효제취물（몰식자산＋몰식자산갑지）조。평개실험약물몰식자유질화유효제취물대구강세균생물막중GTF 활성적영향。결과경몰식자유질급기유효제취물작용후，몰식자유질조24 h 적구강세균생물막중 GTF 활성위（0．0186±0．077），유효제취물조 GTF 활성위（0．0527±0．035），GTF 적활성수도명현억제，여대조조 GTF 활성（0．6601±0．204）상비차이균유통계학의의（P ＜0．05）。결론실험약물몰식자유질급기유효제취물가능통과억제세균생물막중 GTF 활성이발휘방우작용。
Objective The aim of this study is to investigate the effect of Aleppo gall and its active compo-nent on the action of GTF in oral bacteria biofilm and its preventive mechanism.Methods The visible light semiquantitative method has been used to measure biomass GTF (OD620)values so that the effect of Aleppo gall and active component on GTF of oral bacteria biofilm has been discovered.The experiment samples were divided into 3 groups:control group,Aleppo gall group and active component group.Result It was discovered that Aleppo gall and active component were able to inhibit GTF activity of 24 h bacte-rial biofilm (GTF activity of Aleppo gall group is 0.018 6±0.077,GTF activity of active component group is 0.052 7±0.035),compared with control group (GTF activity of control group is 0.6601±0.204),it had statistical significance (P <0.05).Conclusion The effect of Aleppo gall on oral bacterial biofilm not only inhibits bacteria biofilm growth,but also adjusts GTF activity of 24h bacterial biofilm so that it has a very obvious anti-caries effect.