CAJ | 학술논문

目的：探讨有限稀释克隆法培养分离人牙周膜干细胞株的实验方法，并对其生物学特性进行初步研究。方法采用有限稀释克隆法对人牙周膜细胞逐步分离筛选，观察分离出的人牙周膜干细胞形态及克隆形成情况，并对其细胞周期、STRO-1、CD146等标志蛋白的表达进行检测，检测其体外诱导成骨的能力。结果通过有限稀释法获得人牙周膜干细胞克隆具有成体干细胞的形态特征；细胞周期分析呈慢周期性；免疫细胞化学染色证实 STRO-1、CD146及 Vimentin 均为阳性染色，矿化诱导证实该细胞可以表达多种骨蛋白标记物。结论采用有限稀释克隆法分离纯化可以获得成体干细胞所具有的细胞周期、表型及骨向分化能力等特点的牙周膜干细胞株。
목적：탐토유한희석극륭법배양분리인아주막간세포주적실험방법，병대기생물학특성진행초보연구。방법채용유한희석극륭법대인아주막세포축보분리사선，관찰분리출적인아주막간세포형태급극륭형성정황，병대기세포주기、STRO-1、CD146등표지단백적표체진행검측，검측기체외유도성골적능력。결과통과유한희석법획득인아주막간세포극륭구유성체간세포적형태특정；세포주기분석정만주기성；면역세포화학염색증실 STRO-1、CD146급 Vimentin 균위양성염색，광화유도증실해세포가이표체다충골단백표기물。결론채용유한희석극륭법분리순화가이획득성체간세포소구유적세포주기、표형급골향분화능력등특점적아주막간세포주。
Objective To obtain human periodontal ligament stem cell (hPDLSCs)cloning strain by limited dilution method for a preliminary research on its biological characteristics.Methods The limiting dilution method was adopted to separate gradually and screening hPDLSCs cloning from human periodontal liga-ment cells;the osteogenic capability in vitro,morphology and colony formation of the isolated hPDLSCs were observed;STRO-1,CD146 and other signs of protein expression were detected.Results The hP-DLSCs cloning obtained by limiting dilution similar were characteristic of the morphology of adult stem cells;Cell cycle analysis showed a slow cyclicality;STRO-1,CD146 were positively expressed by immu-nocytochemical staining;the cells induced by mineralization can express a variety of markers of bone pro-teins.Conclusion The purification of limited dilution can obtain hPDLSCs with the same features of adult stem cells in terms of cell cycle,phenotypic and osteogenic differentiation capacity.