CAJ | 학술논문

目的：研究伊木萨克片对异常黏液质阳痿病证大鼠性腺轴形态学的影响，探寻其对生殖激素调节作用的形态学基础。方法取90只性功能正常雄性 SD 大鼠，其中10只为正常对照组，余80只为造模组，采用芫荽实＋菠菜实＋湿寒性环境的干预条件建立异常黏液质证候模型，20 w 后从证候模型中筛出阳痿病证模型，并将其分为病证模型组、药物干预组，干预2 w 后，观察下丘脑、垂体和睾丸形态学变化。结果（1）下丘脑：正常组组织结构与细胞形态完好。病证模型组基质疏松，呈网状结构，轻度水肿；超微结构可见，神经胶质细胞基质密度下降，核周隙增宽，线粒体水肿呈空泡样病变，内质网扩张，神经元核膜界线不清，线粒体基质密度下降，粗面内质网扩张。药物干预组得到一定程度修复。（2）垂体：正常组结构完好。病证模型组基质疏松，轻度水肿；超微结构可见，内质网明显扩张，线粒体普遍空泡样变。药物干预组细胞排列紧密，粗面内质网扩张减少，有一定修复。（3）睾丸：正常组结构完好。病证模型组可见生精小管明显变形，各级生精细胞数量明显减少，间质减少；超微结构显示，生精细胞数量减少且排列紊乱，可见有精原细胞、精母细胞核固缩，精子细胞界限模糊不清。药物干预组生精细胞数量明显增多，支持细胞增加，管腔内可见一定数量的精子。结论异常黏液质型阳痿病证大鼠性腺轴形态学可发生明显改变，维药伊木萨克片有促进其修复的作用。
목적：연구이목살극편대이상점액질양위병증대서성선축형태학적영향，탐심기대생식격소조절작용적형태학기출。방법취90지성공능정상웅성 SD 대서，기중10지위정상대조조，여80지위조모조，채용원유실＋파채실＋습한성배경적간예조건건립이상점액질증후모형，20 w 후종증후모형중사출양위병증모형，병장기분위병증모형조、약물간예조，간예2 w 후，관찰하구뇌、수체화고환형태학변화。결과（1）하구뇌：정상조조직결구여세포형태완호。병증모형조기질소송，정망상결구，경도수종；초미결구가견，신경효질세포기질밀도하강，핵주극증관，선립체수종정공포양병변，내질망확장，신경원핵막계선불청，선립체기질밀도하강，조면내질망확장。약물간예조득도일정정도수복。（2）수체：정상조결구완호。병증모형조기질소송，경도수종；초미결구가견，내질망명현확장，선립체보편공포양변。약물간예조세포배렬긴밀，조면내질망확장감소，유일정수복。（3）고환：정상조결구완호。병증모형조가견생정소관명현변형，각급생정세포수량명현감소，간질감소；초미결구현시，생정세포수량감소차배렬문란，가견유정원세포、정모세포핵고축，정자세포계한모호불청。약물간예조생정세포수량명현증다，지지세포증가，관강내가견일정수량적정자。결론이상점액질형양위병증대서성선축형태학가발생명현개변，유약이목살극편유촉진기수복적작용。
Objective To study the effects of Yimusake on gonad axis morphological changes of abmormal balgam syndrome with impotence disease rats model induced by Uyghur medicine.Methods Ninety normal mature male SD rats were selected in the study,10 of them were randomly chosen as the control group;other 80 were treated with spinach and coriander diet in a cold and humid environment until the model of abnormal balgam syndrome was established.Rat biological characterization was observed about 20 weeks until establishment of abnormal balgam syndrome model,then through APO erectile experiment and mating experiment,the rats were randomly divided into abnormal balgam syndrome with impotence disease model group and medication disproof group of abnormal balgam syndrome with impotence disease model.After 2 weeks disproof,we detected the morphological changes of hypothalamus,pituitary and tes-tis.Results (1)Hypothalamus:stucture was normal in normal control group.Matrix was loose,mild e-dema,ultrastructure showed,the glial cell matrix density decreased,mitochondria edema was in cavity changes,endoplasmic reticulum expanded,nuclear membrane of neurons was not clear,mitochondrial ma-trix density decreased,rough endoplasmic reticulum expanded.Minor repaired in medication disproof group of abnormal balgam syndrome with impotence disease model.(2)Pituitary:stucture was normal in normal control group.Matrix was loose,mild edema,ultrastructures showed endoplasmic reticulum ex-panded,mitochondria was in cavity changes.Minor repaired in medication disproof group of abnormal bal-gam syndrome with impotence disease model.(3)Testis:stucture was normal in normal control group. Seminiferous ducts were obviously deformed,interstitial decreased,number and layers of sperm cells sig-nificantly reduced.Ultrastructures showed decreased number and disordered arrangement of sperm cells, nuclear pyknosis of spermatagonial cells and spermatocytes,unclear boundaries of sperm cell.Minor re-paired in medication disproof group of abnormal balgam syndrome with impotence disease model.Conclu-sion Significant morphological changes of gonad axis in abnormal balgam syndrome with impotence disease rat model induced by Uygur medicine,and Yimusake can prevent and repair the damages.