CAJ | 학술논문

目的：了解肝细胞癌（HCC）患者外周血单个核细胞（PBMC）、人类白细胞抗原 G mRNA（HLA-G mRNA）的表达水平及其意义。方法收集44例 HCC患者、21例肝硬化患者和40例健康查体者的 PBMC，建立逆转录-实时荧光 PCR相对定量体系检测PBMC HLA-G mRNA相对表达量。结果 HCC组、肝硬化组和健康对照组的 HLA-G mRNA相对表达量分别为1.71±0.39，1.05±0.38和1.01±0.47，HCC组高于肝硬化组和对照组，差异有统计学意义（F＝33.657，P＜0.001）。HLA-G mRNA高表达的 HCC患者生存率低于 HLA-G mRNA低表达者，差异有统计学意义（χ2＝5.972，P＝0.015）。结论 PBMC HLA-G mRNA相对表达量与肝癌发生发展密切相关，检测 PBMC HLA-G mRNA有助于肝癌的诊断与研究。
목적：료해간세포암（HCC）환자외주혈단개핵세포（PBMC）、인류백세포항원 G mRNA（HLA-G mRNA）적표체수평급기의의。방법수집44례 HCC환자、21례간경화환자화40례건강사체자적 PBMC，건립역전록-실시형광 PCR상대정량체계검측PBMC HLA-G mRNA상대표체량。결과 HCC조、간경화조화건강대조조적 HLA-G mRNA상대표체량분별위1.71±0.39，1.05±0.38화1.01±0.47，HCC조고우간경화조화대조조，차이유통계학의의（F＝33.657，P＜0.001）。HLA-G mRNA고표체적 HCC환자생존솔저우 HLA-G mRNA저표체자，차이유통계학의의（χ2＝5.972，P＝0.015）。결론 PBMC HLA-G mRNA상대표체량여간암발생발전밀절상관，검측 PBMC HLA-G mRNA유조우간암적진단여연구。
Objective To investigate the expression level of peripheral blood mononuclear cell(PBMC)human leucocyte anti-gen G(HLA-G)in patients with hepatocellular carcinoma(HCC).Methods The HLA-G mRNA in PBMC from 44 patients with HCC,21 patients with liver cirrhosis and 40 healthy subjects were measured by reverse transcription real time fluores-cent relative quantitative PCR.Results HLA-G mRNA expression level were 1.71±0.39,1.05±0.38 and 1.01±0.47 in HCC group,liver cirrhosis group and healthy control group respectively.HCC group was higher than the other two groups, the difference was statistically significant (F=33.657,P<0.001).The survival rate of HCC patients in HLA-G mRNA high-expression group was lower than HLA-G mRNA low-expression group,the difference was statistically significant (χ2=5.972,P=0.015).Conclusion PBMC HLA-G mRNA in HCC was closely correlated with tumorigenesis.It can proviede a novel diagnosis and research tool for HCC.