CAJ | 학술논문

目的：研究单纯性先天性心脏病（CHD）患者血浆中 miR-324-5p表达变化，评价其在 CHD诊断方面的临床应用价值。方法收集2012年6月～2013年2月76例CHD患儿及13例健康正常对照血清标本，应用 Real-time PCR检测血浆中miR-324-5p表达变化，通过受试者工作曲线（ROC），评价血浆 miR-324-5p在 CHD诊断方面的临床应用价值。结果与正常对照相比，单纯性CHD患者外周血浆miR-324-5p表达升高（P＝0.032）；受试者工作曲线（ROC）分析显示，曲线下面积（AUC）为0.731，最佳诊断临界值为0.1161，敏感度为64.5％，特异度为84.6％。结论血浆 miR-324-5p作为CHD早期排除诊断的生物学标志物并具有一定的临床应用价值，但还需要与其它指标联合应用。
목적：연구단순성선천성심장병（CHD）환자혈장중 miR-324-5p표체변화，평개기재 CHD진단방면적림상응용개치。방법수집2012년6월～2013년2월76례CHD환인급13례건강정상대조혈청표본，응용 Real-time PCR검측혈장중miR-324-5p표체변화，통과수시자공작곡선（ROC），평개혈장 miR-324-5p재 CHD진단방면적림상응용개치。결과여정상대조상비，단순성CHD환자외주혈장miR-324-5p표체승고（P＝0.032）；수시자공작곡선（ROC）분석현시，곡선하면적（AUC）위0.731，최가진단림계치위0.1161，민감도위64.5％，특이도위84.6％。결론혈장 miR-324-5p작위CHD조기배제진단적생물학표지물병구유일정적림상응용개치，단환수요여기타지표연합응용。
Objective To analyze expression of miR-324-5p in plasma of CHD patients by real-time PCR and identification of early diagnosis,for CHD.Methods In 2012 June to 2013 February 76 patients and 13 normal controls who were included in the study had measurement of plasma expression levels of miR-324-5p by real-time PCR.MedCalc 13.0 software were used for statistical analysis and comprehensive evaluation of miR-324-5p in CHD disease for diagnosis.Results Analysis of re-ceiver operating curve (ROC)showed that area under the curve (AUC)was 0.731 and best diagnostic threshold was 0.116 1.The sensitivity was 64.5% and specificity of 84.6%.Conclusion Circulating miR-324-5p as a biomarker for early diagno-sis of CHD has some-extent clinical value,but needs combined with other medical indicators.