CAJ | 학술논문

目的：探讨胃癌患者的血清乳酸脱氢酶（lactate dehydrogenase，LDH）水平及癌组织中该基因的表达与患者预后的相关性。方法2012～2013年经某院病理确诊的胃癌患者的术前血清标本及癌组织标本各60例，检测血清标本的LDH水平，并使用实时荧光定量反转录PCR的方法检测60例患者的正常胃组织、癌组织及淋巴结组织中 LDH基因的表达。最后，将血清 LDH水平和胃癌组织中 LDH基因的差异表达与患者的临床病理特征进行相关性分析，其中多样本比较采用单因素方差分析，两样本比较使用独立t检验。结果胃癌患者血清LDH为340.89±10.67 IU/ml，显著高于对照组（t＝24.7，P＜0.01）；胃癌组织及淋巴结组织中 LDH的相对表达量分别是正常组织的3.39及2.35倍。与临床病理特征的相关性分析提示血清 LDH的升高与 pTNM分期及是否有淋巴结转移相关（t＝5.2，4.8，P 值均＜0.01），胃癌组织LDH的高表达与肿瘤大小、pTNM分期及是否有淋巴结转移相关（t＝18.2，15.3，F＝7.2，P值均＜0.01）。结论胃癌患者的血清 LDH水平及其组织中 LDH基因表达显著升高，其高表达与患者预后相关。检测血清 LDH 水平及胃癌组织中LDH表达可能成为评估胃癌患者预后的潜在指标。
목적：탐토위암환자적혈청유산탈경매（lactate dehydrogenase，LDH）수평급암조직중해기인적표체여환자예후적상관성。방법2012～2013년경모원병리학진적위암환자적술전혈청표본급암조직표본각60례，검측혈청표본적LDH수평，병사용실시형광정량반전록PCR적방법검측60례환자적정상위조직、암조직급림파결조직중 LDH기인적표체。최후，장혈청 LDH수평화위암조직중 LDH기인적차이표체여환자적림상병리특정진행상관성분석，기중다양본비교채용단인소방차분석，량양본비교사용독립t검험。결과위암환자혈청LDH위340.89±10.67 IU/ml，현저고우대조조（t＝24.7，P＜0.01）；위암조직급림파결조직중 LDH적상대표체량분별시정상조직적3.39급2.35배。여림상병리특정적상관성분석제시혈청 LDH적승고여 pTNM분기급시부유림파결전이상관（t＝5.2，4.8，P 치균＜0.01），위암조직LDH적고표체여종류대소、pTNM분기급시부유림파결전이상관（t＝18.2，15.3，F＝7.2，P치균＜0.01）。결론위암환자적혈청 LDH수평급기조직중 LDH기인표체현저승고，기고표체여환자예후상관。검측혈청 LDH 수평급위암조직중LDH표체가능성위평고위암환자예후적잠재지표。
Objective To explore the association between the level of lactate dehydrogenase (LDH)in gastric cancer patients’ serum or the relative expression of LDH in their tissues and the patients’prognosis.Methods 60 specimens of gastric canc-er patients who confirmed by pathological diagnosis were collected from 2012 to 2013,include the serum specimens and tis-sue specimens.Their serum level of LDH were detected,and the relative expressions level of LDH in the sample of normal tissues,gastric cancer tissues and metastatic lymph nodes were quantify by real-time fluorescence quantitative reverse tran-scription-PCR (Realtime-PCR).Finally,the relationship between the expression of LDH and clinical pathologic features were analyzed by independent t-test or anova statistics.Results The serum level of LDH was highly increased than control group (340.89±10.67 IU/ml,t=24.7,P<0.01);the relative gene expression of LDH in normal tissue,human gastric cancer and metastatic lymph node were 1.0,3.39 and 2.35.The result suggests the serum level of LDH were associated with pTNM stage and lymph node metastases (t=5.2,4.8,P<0.01).The relative gene expression of LDH in gastric canc-er tissues were associated with tumor sizes,pTNM stage and lymph node metastases (t=18.2,15.3,P<0.01 and F=7.2, P<0.01).Conclusion The serum LDH level and the expression of LDH in cancer tissue were significantly increased,and their expression were correlated with the patient’s prognosis.The serum level of LDH and the expression of LDH in gastric cancer tissue may be potential indicator to evaluated the prognosis of the patient with gastric cancer.