现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2014年
5期
40-41,45
,共3页
赵英伦%胡溪源%李翠萍%周怡%李洪涛%何敏%罗蓉
趙英倫%鬍溪源%李翠萍%週怡%李洪濤%何敏%囉蓉
조영륜%호계원%리취평%주이%리홍도%하민%라용
肺结核%caspase-3%凋亡
肺結覈%caspase-3%凋亡
폐결핵%caspase-3%조망
tuberculosis%caspase-3%apoptosis
目的:观察肺结核患者血清 caspase-3活性。方法选取广西医科大学第一附属医院感染科2010年12月住院肺结核患者38例,其中肺结核初治结核杆菌痰涂片阳性13例、肺结核初治结核杆菌痰涂片阴性15例、肺结核复治耐药10例,于入院次日采集外周静脉血,同时选取健康体检者22例为对照组采用分光光度法检测肺结核患者血清 caspase-3酶活力单位,即 caspase-3催化产生的4-硝基苯胺(pNA)的吸光度(A)。结果肺结核初治结核杆菌痰涂片阳性组、肺结核初治结核杆菌痰涂片阴性组、肺结核复治耐药组、正常对照组血清 caspase-3酶活力单位分别为37.15±5.06,50.38±6.29,45.32±3.32和23.89±2.29。各组之间 caspase-3活性差异具有统计学意义(F=10.68,P<0.01)。与正常对照组相比,肺结核初治涂阳组(q=3.51,P=0.016)、肺结核初治涂阴组(q=7.5,P<0.001)、肺结核复治耐药组(q=5.34,P=0.001) caspase-3活性差异具有统计学意义。肺结核患者各亚组(耐药组、涂阳组、涂阴组)之间 caspase-3活性差异无统计学意义(P>0.05)。各组之间性别构成比[χ2=0.38(DL=3),P=0.94]差异无统计学意义;各组之间年龄差异亦无统计学意义(F=0.82,P=0.49)。结论 caspase-3与肺结核发生和发展的过程密切相关,其机制可能通过 caspase-3的激活促进了病变细胞的细胞凋亡从而产生机体抗结核的免疫过程。
目的:觀察肺結覈患者血清 caspase-3活性。方法選取廣西醫科大學第一附屬醫院感染科2010年12月住院肺結覈患者38例,其中肺結覈初治結覈桿菌痰塗片暘性13例、肺結覈初治結覈桿菌痰塗片陰性15例、肺結覈複治耐藥10例,于入院次日採集外週靜脈血,同時選取健康體檢者22例為對照組採用分光光度法檢測肺結覈患者血清 caspase-3酶活力單位,即 caspase-3催化產生的4-硝基苯胺(pNA)的吸光度(A)。結果肺結覈初治結覈桿菌痰塗片暘性組、肺結覈初治結覈桿菌痰塗片陰性組、肺結覈複治耐藥組、正常對照組血清 caspase-3酶活力單位分彆為37.15±5.06,50.38±6.29,45.32±3.32和23.89±2.29。各組之間 caspase-3活性差異具有統計學意義(F=10.68,P<0.01)。與正常對照組相比,肺結覈初治塗暘組(q=3.51,P=0.016)、肺結覈初治塗陰組(q=7.5,P<0.001)、肺結覈複治耐藥組(q=5.34,P=0.001) caspase-3活性差異具有統計學意義。肺結覈患者各亞組(耐藥組、塗暘組、塗陰組)之間 caspase-3活性差異無統計學意義(P>0.05)。各組之間性彆構成比[χ2=0.38(DL=3),P=0.94]差異無統計學意義;各組之間年齡差異亦無統計學意義(F=0.82,P=0.49)。結論 caspase-3與肺結覈髮生和髮展的過程密切相關,其機製可能通過 caspase-3的激活促進瞭病變細胞的細胞凋亡從而產生機體抗結覈的免疫過程。
목적:관찰폐결핵환자혈청 caspase-3활성。방법선취엄서의과대학제일부속의원감염과2010년12월주원폐결핵환자38례,기중폐결핵초치결핵간균담도편양성13례、폐결핵초치결핵간균담도편음성15례、폐결핵복치내약10례,우입원차일채집외주정맥혈,동시선취건강체검자22례위대조조채용분광광도법검측폐결핵환자혈청 caspase-3매활력단위,즉 caspase-3최화산생적4-초기분알(pNA)적흡광도(A)。결과폐결핵초치결핵간균담도편양성조、폐결핵초치결핵간균담도편음성조、폐결핵복치내약조、정상대조조혈청 caspase-3매활력단위분별위37.15±5.06,50.38±6.29,45.32±3.32화23.89±2.29。각조지간 caspase-3활성차이구유통계학의의(F=10.68,P<0.01)。여정상대조조상비,폐결핵초치도양조(q=3.51,P=0.016)、폐결핵초치도음조(q=7.5,P<0.001)、폐결핵복치내약조(q=5.34,P=0.001) caspase-3활성차이구유통계학의의。폐결핵환자각아조(내약조、도양조、도음조)지간 caspase-3활성차이무통계학의의(P>0.05)。각조지간성별구성비[χ2=0.38(DL=3),P=0.94]차이무통계학의의;각조지간년령차이역무통계학의의(F=0.82,P=0.49)。결론 caspase-3여폐결핵발생화발전적과정밀절상관,기궤제가능통과 caspase-3적격활촉진료병변세포적세포조망종이산생궤체항결핵적면역과정。
Objective To explore serum caspase-3 activity in pulmonary tuberculosis patients.Methods 38 tuberculosis pa-tients in Infectious Disease Department in the First Affiliated Hospital of Guangxi Medical University were recruited and the peripheral venous blood was collected in December,2010.Among them,sputum smear-positive patients which were first treated were 13,sputum smear-negative patients which were first treated were 15,10 cases were drug resistance.In addition, 22 unrelated healthy individuals from a routine health survey were enrolled as controls.Spectrophotometry was used to de-tect serum caspase-3 activity in tuberculosis patients,which was absorbancy-A of 4-nitroaniline generated by caspase-3.Re-sults The activity of caspase-3 in sputum smear-positive patients,sputum smear-negative patients,drug resistance patients, and healthy controls were 37.15±5.06,50.38±6.29,45.32±3.32 and 23.89±2.29,respectively.The caspase-3 activity was significantly different from each other (F=10.68,P<0.01).Compared with the healthy controls,the caspase-3 activity in sputum smear-positive patients (q=3.51,P=0.016),sputum smear-negative patients (q=7.5,P<0.001),drug resist-ance patients (q=5.34,P=0.001)was significantly different.There was no statistical difference among sputum smear-posi-tive patients,sputum smear-negative patients and drug resistance pulmonary tuberculosis patients (P>0.05).In the same way,there was no significant difference in male/female ratio[χ2=0.38 (DL=3),P=0.94]and mean age (F=0.82,P=0.49)among the four group.Conclusion Caspase-3 closely associated with the development of tuberculosis occurrence, which might promote apoptosis of cells through the activation of caspase-3 and produce the body immune process of tubercu-losis.