CAJ | 학술논문

目的：探讨T细胞免疫球蛋白黏蛋白-3（TIM-3）在原发性舍格伦综合征（pSS）发病机制中的作用。方法收集2012年10月～2013年7月期间上海长征医院和长海医院门诊及住院 pSS患者33例，同期纳入健康对照者33例。采用实时荧光定量逆转录-聚合酶链反应（RT-PCR）方法检测33例 pSS患者及33例健康对照组外周血单个核细胞（PBMCs）中TIM-3的相对表达量，并分析TIM-3与 pSS患者特征性实验室指标（血清 RF，CRP，抗 SSA和抗 SSB抗体）的相关性；对12名接受2个月糖皮质激素治疗的患者进行随访，分析TIM-3 mRNA在 pSS患者治疗前后的表达变化情况。结果pSS患者外周血 PBMCs 中 TIM-3 mRNA 的表达较健康对照组显著升高（0.55±0.12 vs 0.13±0.10，t＝15.44，P＜0.0001），但在抗 SSA和抗 SSB抗体阳性和阴性患者之间，TIM-3水平差异无统计学意义（0.45±0.21 vs 0.54±0.31；0.46±0.15 vs 0.51±0.24；P值均＞0.05）。进一步分析发现，pSS患者外周血 PBMCs 中 TIM-3 mRNA的表达与血清RF，CRP水平呈正相关（R＝0.5585，0.4147；P值均＜0.05）；12名 pSS患者经过糖皮质激素治疗后，TIM-3的表达较治疗前下降（0.62±0.10 vs 0.38±0.13，t＝5.07，P＜0.05）。结论 TIM-3可能参与了pSS的发病机制，是pSS潜在的标志物。
목적：탐토T세포면역구단백점단백-3（TIM-3）재원발성사격륜종합정（pSS）발병궤제중적작용。방법수집2012년10월～2013년7월기간상해장정의원화장해의원문진급주원 pSS환자33례，동기납입건강대조자33례。채용실시형광정량역전록-취합매련반응（RT-PCR）방법검측33례 pSS환자급33례건강대조조외주혈단개핵세포（PBMCs）중TIM-3적상대표체량，병분석TIM-3여 pSS환자특정성실험실지표（혈청 RF，CRP，항 SSA화항 SSB항체）적상관성；대12명접수2개월당피질격소치료적환자진행수방，분석TIM-3 mRNA재 pSS환자치료전후적표체변화정황。결과pSS환자외주혈 PBMCs 중 TIM-3 mRNA 적표체교건강대조조현저승고（0.55±0.12 vs 0.13±0.10，t＝15.44，P＜0.0001），단재항 SSA화항 SSB항체양성화음성환자지간，TIM-3수평차이무통계학의의（0.45±0.21 vs 0.54±0.31；0.46±0.15 vs 0.51±0.24；P치균＞0.05）。진일보분석발현，pSS환자외주혈 PBMCs 중 TIM-3 mRNA적표체여혈청RF，CRP수평정정상관（R＝0.5585，0.4147；P치균＜0.05）；12명 pSS환자경과당피질격소치료후，TIM-3적표체교치료전하강（0.62±0.10 vs 0.38±0.13，t＝5.07，P＜0.05）。결론 TIM-3가능삼여료pSS적발병궤제，시pSS잠재적표지물。
Objective To explore the expression of T cell immunoglobulin and mucin-3(TIM-3)in peripheral blood mononu-clear cells from patients with primary Sj¨ogren’s syndrome(pSS)and its clinical significance.Methods Enrolled 33 pSS pa-tients from Shanghai Changzheng Hospital and Changhai Hospital in October 2012 and July 2013.The relative expression of TIM-3 in the peripheral blood mononuclear cells(PBMC)in 33 patients and 33 healthy individuals was detected by RT-PCR. The association between TIM-3 mRNA level and clinical characteristics were analyzed,for example,serum RF,CRP and anti-SSA/SSB antibody.1 2 of the pSS patients were followed up and their TIM-3 mRNA level was determined after glucocorti-coid treatment for 2 months.Results The expression of TIM-3 in PBMCs from patients with pSS was increased significantly than that in healthy individuals (0.55±0.12 vs 0.13±0.10,t=15.44,P<0.000 1),but its expression between anti-SSA/anti-SSB positive and negative patients were not statistical difference (0.45±0.21 vs 0.54±0.31;0.46±0.15 vs 0.51± 0.24).The TIM-3 mRNA expression in pSS patients was positively correlated with serum RF and CRP (R=0.558 5,R=0.414 7,P>0.05),but was not associated the anti-SSA/SSB antibody (P=0.298 2).TIM-3 mRNA level was decreased in the patients who were treated with glucocorticoids for 2 months (0.62±0.10 vs 0.38±0.13,t=5.07,P<0.05).Conclusion TIM-3 may play a role in the pathogenesis of pSS and it may be regarded as a biomarker to pSS.