中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2014年
13期
56-58
,共3页
慢性阻塞性肺疾病%CCR6%CCR7
慢性阻塞性肺疾病%CCR6%CCR7
만성조새성폐질병%CCR6%CCR7
COPD%CCR6%CCR7
目的：了解树突状细胞的趋化因子受体 CCR6、CCR7在 COPD 大鼠外周血和支气管肺泡灌洗液（BALF）中的变化。方法：选用20只健康 Wistar 大鼠，随机分为正常对照组（10只）、COPD 模型组（10只），采用两次气道内注入细菌内毒素脂多糖（LPS）和连续被动吸烟4周的方法建立 COPD 大鼠模型。第28天处死两组大鼠。取大鼠肺脏组织用石蜡包埋 HE 染色以观察肺组织病理学改变，酶联免疫法（ELISA）检测 BALF 及外周血中 CCR6、CCR7的含量。结果：COPD 模型组大鼠一般状况较正常对照组大鼠差，体重显著减轻模型组（242.31±14.71）g，对照组（324.18±12.89）g，差异具有统计学意义 P ＜0.05），HE 染色提示慢性阻塞性肺疾病的特征性病理改变。BALF 中 CCR6含量在 COPD 模型组（105.81±2.73）ng／L 明显高于正常对照组（43.57±5.51）ng／L，差异具有高度统计学意义（P ＜0.01），CCR7的含量在 COPD 模型组（10.40±3.07）ng／L 低于正常对照组（41.73±7.40）ng／L，差异具有高度统计学意义（P ＜0.01）。两组大鼠外周血中 CCR6含量模型组（264.89±45.34）ng／L，对照组（311.12±29.78）ng／L、CCR7含量模型组（73.77±29.71）ng／L，对照组（56.42±15.30）ng／L 均无统计学差异（P ＞0.05）。结论：COPD 大鼠肺组织病理符合人 COPD 特征性病理改变，提示 COPD 大鼠模型建立成功。树突状细胞趋化因子受体 CCR6在 COPD 大鼠肺部明显增多，而成熟树突状细胞趋化因子受体 CCR7在 COPD 大鼠肺部减少。
目的：瞭解樹突狀細胞的趨化因子受體 CCR6、CCR7在 COPD 大鼠外週血和支氣管肺泡灌洗液（BALF）中的變化。方法：選用20隻健康 Wistar 大鼠，隨機分為正常對照組（10隻）、COPD 模型組（10隻），採用兩次氣道內註入細菌內毒素脂多糖（LPS）和連續被動吸煙4週的方法建立 COPD 大鼠模型。第28天處死兩組大鼠。取大鼠肺髒組織用石蠟包埋 HE 染色以觀察肺組織病理學改變，酶聯免疫法（ELISA）檢測 BALF 及外週血中 CCR6、CCR7的含量。結果：COPD 模型組大鼠一般狀況較正常對照組大鼠差，體重顯著減輕模型組（242.31±14.71）g，對照組（324.18±12.89）g，差異具有統計學意義 P ＜0.05），HE 染色提示慢性阻塞性肺疾病的特徵性病理改變。BALF 中 CCR6含量在 COPD 模型組（105.81±2.73）ng／L 明顯高于正常對照組（43.57±5.51）ng／L，差異具有高度統計學意義（P ＜0.01），CCR7的含量在 COPD 模型組（10.40±3.07）ng／L 低于正常對照組（41.73±7.40）ng／L，差異具有高度統計學意義（P ＜0.01）。兩組大鼠外週血中 CCR6含量模型組（264.89±45.34）ng／L，對照組（311.12±29.78）ng／L、CCR7含量模型組（73.77±29.71）ng／L，對照組（56.42±15.30）ng／L 均無統計學差異（P ＞0.05）。結論：COPD 大鼠肺組織病理符閤人 COPD 特徵性病理改變，提示 COPD 大鼠模型建立成功。樹突狀細胞趨化因子受體 CCR6在 COPD 大鼠肺部明顯增多，而成熟樹突狀細胞趨化因子受體 CCR7在 COPD 大鼠肺部減少。
목적：료해수돌상세포적추화인자수체 CCR6、CCR7재 COPD 대서외주혈화지기관폐포관세액（BALF）중적변화。방법：선용20지건강 Wistar 대서，수궤분위정상대조조（10지）、COPD 모형조（10지），채용량차기도내주입세균내독소지다당（LPS）화련속피동흡연4주적방법건립 COPD 대서모형。제28천처사량조대서。취대서폐장조직용석사포매 HE 염색이관찰폐조직병이학개변，매련면역법（ELISA）검측 BALF 급외주혈중 CCR6、CCR7적함량。결과：COPD 모형조대서일반상황교정상대조조대서차，체중현저감경모형조（242.31±14.71）g，대조조（324.18±12.89）g，차이구유통계학의의 P ＜0.05），HE 염색제시만성조새성폐질병적특정성병리개변。BALF 중 CCR6함량재 COPD 모형조（105.81±2.73）ng／L 명현고우정상대조조（43.57±5.51）ng／L，차이구유고도통계학의의（P ＜0.01），CCR7적함량재 COPD 모형조（10.40±3.07）ng／L 저우정상대조조（41.73±7.40）ng／L，차이구유고도통계학의의（P ＜0.01）。량조대서외주혈중 CCR6함량모형조（264.89±45.34）ng／L，대조조（311.12±29.78）ng／L、CCR7함량모형조（73.77±29.71）ng／L，대조조（56.42±15.30）ng／L 균무통계학차이（P ＞0.05）。결론：COPD 대서폐조직병리부합인 COPD 특정성병리개변，제시 COPD 대서모형건립성공。수돌상세포추화인자수체 CCR6재 COPD 대서폐부명현증다，이성숙수돌상세포추화인자수체 CCR7재 COPD 대서폐부감소。
Objective To investigate expressions of CCR6 and CCR7 in peripheral blood and BALF of rats with COPD.Methods 20 Wistar rats were randomized into normal control group and COPD model group.COPD was induced by established cigarette smoke inhalation about four weeks and intratracheal 200μg/200μL of LPS solution twice totally.All animals were killed at the 28th day.Then pathomorphology of rats’lung and bronchiole were investigated by HE staining.The contents of CCR6 and CCR7 in the BALF were ana-lyzed with ELISA,so were those in the peripheral blood.Results Rats in the COPD model group expressed lassitude and less activity, and their weight was lower than the normal control group with a significant difference (P <0.05).The HE staining result suggest that the changes of pathology of the COPD model at 28 -day were the same as those of COPD patients.The contents of CCR6 and CCR7 in rats’peripheral blood were not significantly different between the two groups.The content of CCR6 in BALF about the COPD model group was significantly increased compared with the controls (P <0.01).The content of CCR7 in BALF about the COPD model group was significantly reduced compared with the controls (P <0.01).Conclusion:The COPD rats model was established successfully by this combined methods.The expressions of CCR6 are increased in the lungs of rats with COPD,but those of CCR7 are decreased.
