CAJ | 학술논문

目的：研究男性不育患者精子形态与精浆锌和精子顶体酶活性关系。方法455例男性不育患者分别根据年龄和精子正常形态百分率分成5组和8组，采用 Diff-Quik 染色法、改良 PRA 法和改良 Kennedy 法分别进行精子形态、精浆锌和精子顶体酶活性的检测。结果各年龄组的正常形态精子百分率和精浆锌浓度差异无统计学意义（P ＞0．05），精子顶体酶活性在36～50岁年龄段显著降低（P ＜0．05），其他年龄段精子顶体酶活性差异无统计学意义（P ＞0．05）。在精子正常形态百分率不同的8组中，年龄差异无统计学意义，精浆锌总量差异无统计学意义，精子顶体酶活性随着精子正常形态百分率降低而降低（r ＝0．93，P ＜0．01）。结论精子正常形态百分率不受年龄和精浆锌的影响，与精子顶体酶活性呈正相关。
목적：연구남성불육환자정자형태여정장자화정자정체매활성관계。방법455례남성불육환자분별근거년령화정자정상형태백분솔분성5조화8조，채용 Diff-Quik 염색법、개량 PRA 법화개량 Kennedy 법분별진행정자형태、정장자화정자정체매활성적검측。결과각년령조적정상형태정자백분솔화정장자농도차이무통계학의의（P ＞0．05），정자정체매활성재36～50세년령단현저강저（P ＜0．05），기타년령단정자정체매활성차이무통계학의의（P ＞0．05）。재정자정상형태백분솔불동적8조중，년령차이무통계학의의，정장자총량차이무통계학의의，정자정체매활성수착정자정상형태백분솔강저이강저（r ＝0．93，P ＜0．01）。결론정자정상형태백분솔불수년령화정장자적영향，여정자정체매활성정정상관。
Objective To investigate the relationship of sperm morphology with seminal plasma zinc and sperm acrosin activity in male infertile patients.Methods 455 patients with male infertility were divided into 5 groups and 8 groups according to age and sperm morphology respectively.The sperm morphology,seminal plasma zinc and sperm acrosin activity were detected by the Diff-Quik stain,modified 5-Br-PAPS and modified Kennedy methods respectively.Results The percentages of morphologically normal sperm and seminal plasma zinc concentration in the infertile men groups were not different in various age groups(P >0.05 ).The sperm acrosin activity in the age group of 36-50 years was remarkably decreased (P <0.05),while which in other age groups had no statistical difference(P >0.05).Seminal plasma zinc concentration in the infertile men groups were not different in the 8 groups (P >0.05 ).Sperm acrosin activity was decreased with the percentage decrease of morphologically normal sperm (r =0.93,P <0.01).Conclusion The percentage of morphologically normal sperm is not affectd by the age and the seminal plasma zinc,which is positively correlated with the sperm acrosin activity.