CAJ | 학술논문

目的：比较女性宫颈正常细胞与不典型鳞状细胞（atypical squamous cells of undetermined significance，ASC-US）中人乳头瘤病毒（human papillomavirus，HPV）感染的基因型分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对1000例宫颈正常细胞和229例宫颈 ASC-US 标本进行23种 HPV 基因分型检测。结果1000例宫颈正常细胞中检出 HPV 阳性感染者106例，总的 HPV 感染率为10．60％（106/1000），其一重感染率为9．30％（93/1000），多重感染率为1．30％（13/1000）；229例宫颈 ASC-US 中检出 HPV 阳性感染者116例，总的 HPV 感染率为50．66％（116/229），其一重感染率为34．06％（78/229），多重感染率为16．59％（38/229）。两组 HPV 总阳性率、一重和多重感染率比较差异有统计学意义（P ＜0．05）。结论HPV16、18、33、42、43、52、58型是宫颈正常细胞及 ASC-US 中最主要的型别，基因扩增结合基因芯片检测技术可应用于宫颈细胞的 HPV 分型检测，有利于在临床上对宫颈 ASC-US 行进一步分流管理，对宫颈癌的防控具有重要意义。
목적：비교녀성궁경정상세포여불전형린상세포（atypical squamous cells of undetermined significance，ASC-US）중인유두류병독（human papillomavirus，HPV）감염적기인형분포정황급기림상의의。방법채용기인확증결합기인심편기술대1000례궁경정상세포화229례궁경 ASC-US 표본진행23충 HPV 기인분형검측。결과1000례궁경정상세포중검출 HPV 양성감염자106례，총적 HPV 감염솔위10．60％（106/1000），기일중감염솔위9．30％（93/1000），다중감염솔위1．30％（13/1000）；229례궁경 ASC-US 중검출 HPV 양성감염자116례，총적 HPV 감염솔위50．66％（116/229），기일중감염솔위34．06％（78/229），다중감염솔위16．59％（38/229）。량조 HPV 총양성솔、일중화다중감염솔비교차이유통계학의의（P ＜0．05）。결론HPV16、18、33、42、43、52、58형시궁경정상세포급 ASC-US 중최주요적형별，기인확증결합기인심편검측기술가응용우궁경세포적 HPV 분형검측，유리우재림상상대궁경 ASC-US 행진일보분류관리，대궁경암적방공구유중요의의。
Objective To compare the distribution situation of human papillomavirus(HPV)infective genotypes in normal cells and atypical squamous cells of undetermined significance(ASC-US)in uterine cervix and its clinical significance.Methods The pol-ymerase chain reaction(PCR)combined with the gene-chips technology were adopted to detect 23 kinds of HPV genotype from 1 000 cases of normal cells specimens and 229 cases of ASC-US specimens.Results 106 cases of HPV-positive infection were de-tected from 1 000 cases of normal cells with the total HPV infection rate of 10.60%(106/1 000),in which the single genotype in-fection rate was 9.30%(93/1 000)and the multiple genotypes infection rate was 1.30%(13/1 000);116 cases of HPV-positive in-fection were detected from 229 cases of cervial ASC-US specimens with the total HPV positive rate was 50.66% (116/229 ),in which the single genotype infection rate was 34.06%(78/229)and the multiple genotypes infection rate was 16.59%(38/229).The total HPV positive rates,single and multiple genotype infection had statistically significantly differences between the two groups(P<0.05).Conclusion The HPV types 16,18,33,42,43,52,58 are the predominant genotypes in normal cervical cells and ASC-US. PCR combined with the gene-chip technology can be used in the HPV genotype detect in cervical cells,conduces to perform the fur-ther distribution management on ASC-US and has the important significance to prevention and control of cervical cancer.