国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
13期
1678-1680
,共3页
黄洁雯%郭晓奎%李擎天
黃潔雯%郭曉奎%李擎天
황길문%곽효규%리경천
抗菌肽%葡萄球菌,金黄色%葡萄球菌,表皮
抗菌肽%葡萄毬菌,金黃色%葡萄毬菌,錶皮
항균태%포도구균,금황색%포도구균,표피
antimicrobial peptide%staphylococcus aureus%staphylococcus epidermidis
目的:探讨抗菌肽人β防御素3融合糖类结合域(hBD3-CBD)对金黄色葡萄球菌 N315、表皮葡萄球菌35984的抑制作用。方法以直接杀伤和分子生物学方法检测 hBD3-CBD 对金黄色葡萄球菌 N315和表皮葡萄球菌35984菌株的抑制作用以及对细菌关键基因表达的影响。结果直接杀菌作用显示,抗菌肽 hBD3以及 hBD3-CBD 对金黄色葡萄球菌 N315、表皮葡萄球菌35984均有显著的抑制作用;hBD3-CBD 的抑制作用强于 hBD3;hBD3-CBD 抑制作用的稳定性亦强于 hBD3。在金黄色葡萄球菌 N315、表皮葡萄球菌35984的关键基因表达检测中,hBD3-CBD 对葡萄球菌的 agr 和 mecA 基因表达有显著抑制作用,还抑制表皮葡萄球菌 icaA 基因的表达和促进 icaR 基因表达,这说明 hBD3-CBD 能够抑制表皮葡萄球菌的生物膜形成。结论抗菌肽的融合策略对于改善抗菌肽的抑菌效能意义重大,并为其未来的应用带来更多希望。
目的:探討抗菌肽人β防禦素3融閤糖類結閤域(hBD3-CBD)對金黃色葡萄毬菌 N315、錶皮葡萄毬菌35984的抑製作用。方法以直接殺傷和分子生物學方法檢測 hBD3-CBD 對金黃色葡萄毬菌 N315和錶皮葡萄毬菌35984菌株的抑製作用以及對細菌關鍵基因錶達的影響。結果直接殺菌作用顯示,抗菌肽 hBD3以及 hBD3-CBD 對金黃色葡萄毬菌 N315、錶皮葡萄毬菌35984均有顯著的抑製作用;hBD3-CBD 的抑製作用彊于 hBD3;hBD3-CBD 抑製作用的穩定性亦彊于 hBD3。在金黃色葡萄毬菌 N315、錶皮葡萄毬菌35984的關鍵基因錶達檢測中,hBD3-CBD 對葡萄毬菌的 agr 和 mecA 基因錶達有顯著抑製作用,還抑製錶皮葡萄毬菌 icaA 基因的錶達和促進 icaR 基因錶達,這說明 hBD3-CBD 能夠抑製錶皮葡萄毬菌的生物膜形成。結論抗菌肽的融閤策略對于改善抗菌肽的抑菌效能意義重大,併為其未來的應用帶來更多希望。
목적:탐토항균태인β방어소3융합당류결합역(hBD3-CBD)대금황색포도구균 N315、표피포도구균35984적억제작용。방법이직접살상화분자생물학방법검측 hBD3-CBD 대금황색포도구균 N315화표피포도구균35984균주적억제작용이급대세균관건기인표체적영향。결과직접살균작용현시,항균태 hBD3이급 hBD3-CBD 대금황색포도구균 N315、표피포도구균35984균유현저적억제작용;hBD3-CBD 적억제작용강우 hBD3;hBD3-CBD 억제작용적은정성역강우 hBD3。재금황색포도구균 N315、표피포도구균35984적관건기인표체검측중,hBD3-CBD 대포도구균적 agr 화 mecA 기인표체유현저억제작용,환억제표피포도구균 icaA 기인적표체화촉진 icaR 기인표체,저설명 hBD3-CBD 능구억제표피포도구균적생물막형성。결론항균태적융합책략대우개선항균태적억균효능의의중대,병위기미래적응용대래경다희망。
Objective To explore the inhibition of fusing antimicrobial peptides humanβ-defensin 3 and carbohydrate binding do-main on Staphylococcus aureus N315 and Staphylococcus epidermidis 35984.Methods The direct bactericidal test and other molec-ular biology methods were adopted to detect the inhibition role on Staphylococcus aureus strain N315 and Staphylococcus epidermi-dis strain 35984 and the influence on the key genes expression.Results The direct bactericidal test demonstrated that antimicrobial peptides hBD3 and hBD3-CBD had significantly inhibitory effects on staphylococcus aureus N315 and staphylococcus epidermidis 35984;the inhibitory effects of hBD3-CBD was stronger than that of hBD3;the stability of the inhibitory effects of hBD3-CBD also stronger than that of hBD3.In the key gene expression test,there were significant inhibitions on the agr and mecA gene expressions in Staphylococcus aureus N315 and Staphylococcus epidermidis 35984 by hBD3-CBD.At the same time,hBD3-CBD could inhibit the icaA gene expression and promote icaR gene expression in Staphylococcus epidermidis 35984,which indicated that hBD3-CBD could inhibit the biofilm formation of Staphylococcus epidermidis.Conclusion The fusion strategy of antimicrobial peptide has very important significance for improving the antibacterial efficacy of antimicrobial peptides and brings more hope in their future applications.