CAJ | 학술논문

目的：探讨转化生长因子-β1基因多态性与乙型肝炎病毒（HBV）慢性感染发展至肝细胞癌风险的关系。方法随机纳入120例 HBV 慢性感染患者（病例组）和100例与病例组性别、年龄相匹配的健康者（对照组），并将病例组分为非肝癌组和肝癌组。采用聚合酶链反应-限制性片段长度多态性分析法检测各组 TGF-β1基因多态性（T29C）并结合 TGF-β1 mRNA 水平进行比较分析。结果携带基因型 CC 发生肝癌的风险较携带基因型 TT 显著降低（OR ＝0．317，95％CI ＝0．110～0．913，P ＝0．033；OR＝284，95％CI ＝0．093～0．866，P ＝0．027）。携带等位基因 C 较等位基因 T 发生肝癌的风险显著降低（OR ＝0．570，95％CI ＝0．341～0．953，P ＝0．032；OR＝0．548，95％CI ＝0．320～0．936，P ＝0．028）。肝癌组中携带基因型 CC 的患者 TGF-β1 mRNA 水平较低。结论TGF-β1基因 T29C 多态性可能与 HBV 慢性感染进展至肝细胞癌风险有关。
목적：탐토전화생장인자-β1기인다태성여을형간염병독（HBV）만성감염발전지간세포암풍험적관계。방법수궤납입120례 HBV 만성감염환자（병례조）화100례여병례조성별、년령상필배적건강자（대조조），병장병례조분위비간암조화간암조。채용취합매련반응-한제성편단장도다태성분석법검측각조 TGF-β1기인다태성（T29C）병결합 TGF-β1 mRNA 수평진행비교분석。결과휴대기인형 CC 발생간암적풍험교휴대기인형 TT 현저강저（OR ＝0．317，95％CI ＝0．110～0．913，P ＝0．033；OR＝284，95％CI ＝0．093～0．866，P ＝0．027）。휴대등위기인 C 교등위기인 T 발생간암적풍험현저강저（OR ＝0．570，95％CI ＝0．341～0．953，P ＝0．032；OR＝0．548，95％CI ＝0．320～0．936，P ＝0．028）。간암조중휴대기인형 CC 적환자 TGF-β1 mRNA 수평교저。결론TGF-β1기인 T29C 다태성가능여 HBV 만성감염진전지간세포암풍험유관。
Objective To investigate the relationship between the polymorphism of transforming growth factor-β1 (TGF-β1 ) gene and risk of chronic hepatitis B virus(HBV)infection progressing to hepatocellular carcinoma(HCC).Methods 120 patients with chronic HBV infection(case group)and 100 age-and sex-matched healthy individuals(healthy control group)were randomly enrolled to this study.The polymerase chain reaction-restriction fragment length polymorphism technique was adopted to detect the single nucleotide polymorphism of TGF-β1 gene(T29C),and made the comparative analysis combined with TGF-β1 mRNA level. Results The risk of HCC occurrence in the patients carrying genotype CC was decreased than that in the patients carrying geno-type TT (OR=0.317,95%CI =0.110-0.913,P =0.033;OR=284,95%CI =0.093 -0.866,P =0.027),the risk of HCC in pa-tients carrying allele C was significantly decreased compared with that in the patients carrying allele T(OR =0.570,95%CI =0.341 -0.953,P =0.032;OR=0.548,95%CI =0.320-0.936,P =0.028).In the HCC group,the patients carrying genotype CC had the lower lever of TGF-β1 mRNA.Conclusion TGF-β1 gene polymorphism(T29C)may be related to the risk of chronic HBV infection progressing to HCC.