CAJ | 학술논문

目的：建立测定患儿血清中甲氨蝶呤浓度的方法，用于临床患儿血药浓度监测。方法色谱柱采用岛津原装柱（250 mm×4．6 mm，5μm），流动相为0．025 mol/L 磷酸二氢钠溶液（pH 为5．41）-甲醇（76∶24，V/V），流速1．0 mL/min，检测波长313 nm，柱温35℃，样品经10％高氯酸沉淀蛋白后，取上清液130μL 加10μL 1 mol/L NaOH 后直接进样。结果血浆中甲氨蝶呤的浓度在0．00～4．84μmol/L 和4．84～10．00μmol/L 范围内与峰面积线性关系良好，r 1＝1，r2＝1，平均回收率分别为61．67％和71．83％，日内及日间精密度的 RSD 小于10％和12％（n＝3）。结论所用方法简便快速、准确可靠、灵敏度高、重现性好、线性范围宽和成本低，适于甲氨蝶呤的血药浓度监测。
목적：건립측정환인혈청중갑안접령농도적방법，용우림상환인혈약농도감측。방법색보주채용도진원장주（250 mm×4．6 mm，5μm），류동상위0．025 mol/L 린산이경납용액（pH 위5．41）-갑순（76∶24，V/V），류속1．0 mL/min，검측파장313 nm，주온35℃，양품경10％고록산침정단백후，취상청액130μL 가10μL 1 mol/L NaOH 후직접진양。결과혈장중갑안접령적농도재0．00～4．84μmol/L 화4．84～10．00μmol/L 범위내여봉면적선성관계량호，r 1＝1，r2＝1，평균회수솔분별위61．67％화71．83％，일내급일간정밀도적 RSD 소우10％화12％（n＝3）。결론소용방법간편쾌속、준학가고、령민도고、중현성호、선성범위관화성본저，괄우갑안접령적혈약농도감측。
Objective To establish a HPLC method for determining the serum methotrexate(MTX)concentration in children patients for monitoring the blood drug concentration in clinic.Methods The chromatographic analytical column was the Shimaduz C-18 colum(250 mm×4.6 mm,5 μm),the mobile phase was 0.025 mol/L NaH2 PO4 (pH 5.41)-methanol(76∶24,V/V).The flow rate was 1 .0 mL/min.The detection wavelength was 313 nm.The column temperature was set at 35 ℃.The plasma samples were deposited down the protein with 10% HClO4 ,the supernatant liquid 130 μL added with 1 mol/L NaOH 10 μL was directly injected for determination.Results The mass concentration of MTX in the range of 0.00-4.84 μmol/L and 4.84-10.00 μmol/L showed the good linear relation with the peak area,r 1 =1 ,r2 =1 .The mean recovery rates were 61.67%and 71.83%.Intra-day and inter-day precision were <10% and 12%,respectively(n =3).Conclusion The used method is simple,accurate and reliable with high sensitivity,good reproducibility and wide linearity range,which is suitable for the monitoring blood MTX concentration.