CAJ | 학술논문

目的：建立左股骨中段闭合折骨经皮髓内克氏针固定的标准骨折模型，观察丹参接骨胶囊对SD大鼠骨折愈合过程中局部转化生长因子β1（transforming growth factor beta 1，TGF-β1）mRNA、蛋白表达，通过RT-PCR，ELISA法进行分析转化生长因子β1的表达，探讨丹参接骨胶囊对大鼠骨折愈合的机制。方法：建立闭合性的股骨干骨折髓内固定模型，分别给与相应药物灌胃治疗，在不同时相通过 RT-PCR，ELISA 法进行分析 TGF-β1 mRNA 及蛋白表达。结果：丹参接骨胶囊能促进骨折 SD 大鼠TGF-β1mRNA及蛋白的表达。结论：大鼠股骨中段闭合折骨髓内克氏针固定骨折模型稳定，可操作性强，重复性好。丹参接骨胶囊能促进骨折SD大鼠TGF-β1mRNA及蛋白的表达。
목적：건립좌고골중단폐합절골경피수내극씨침고정적표준골절모형，관찰단삼접골효낭대SD대서골절유합과정중국부전화생장인자β1（transforming growth factor beta 1，TGF-β1）mRNA、단백표체，통과RT-PCR，ELISA법진행분석전화생장인자β1적표체，탐토단삼접골효낭대대서골절유합적궤제。방법：건립폐합성적고골간골절수내고정모형，분별급여상응약물관위치료，재불동시상통과 RT-PCR，ELISA 법진행분석 TGF-β1 mRNA 급단백표체。결과：단삼접골효낭능촉진골절 SD 대서TGF-β1mRNA급단백적표체。결론：대서고골중단폐합절골수내극씨침고정골절모형은정，가조작성강，중복성호。단삼접골효낭능촉진골절SD대서TGF-β1mRNA급단백적표체。
Objectives:To observe the effects of Danshen Jiegu capsule on expression of transforming growth factor beta 1 mRNA and protein in rat fracture healing.To explore the mechanisms of fracture healing in rats by Danshen Jiegu capsule,according to RT-PCR, ELISA methods.Methods:To establish standard fracture model of the midshaft femur closed fracture fixed by Kirschner wires percutaneously.The rats in treatment group were administrated with Danshen Jiegu capsule,while the saline group were treated with equal volume of saline.Specimens of bony calluses were collected at 3,5,7,14,21days after fracture to detect the expression of TGF-β1 by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.Results:The expression of TGF-β1 mRNA was higher than that in saline group.Conclusion:Danshen Jiegu capsule increases the expression TGF-β1 in bony callus of rats with early fracture ,and promotes the fracture healing.