CAJ | 학술논문

通过单因素和 Box-Behnken 设计优化了最佳提取料液比、温度、时间条件,同时比较了最佳工艺条件下的超声辅助提取、传统酶法提取和静置提取的花色苷提取率及花色苷吸收光谱,并采用高效液相色谱(HPLC)分析了3种提取方法对花色苷组分的影响．结果表明：超声辅助提取最佳条件为提取时间119 min,提取温度69．7℃,超声时间45．8 min,稀释倍数7．8,预测得到花色苷的最大提取率为58．83±1．78％．在最优条件下,花色苷的提取率为58．64±0．32％,相对误差仅为0．95％,模型准确度高．超声辅助提取比传统酶法提取的花色苷提取率高12．96％．经 HPLC 分析,超声辅助提取提高了花色苷组分的提取率．
통과단인소화 Box-Behnken 설계우화료최가제취료액비、온도、시간조건,동시비교료최가공예조건하적초성보조제취、전통매법제취화정치제취적화색감제취솔급화색감흡수광보,병채용고효액상색보(HPLC)분석료3충제취방법대화색감조분적영향．결과표명：초성보조제취최가조건위제취시간119 min,제취온도69．7℃,초성시간45．8 min,희석배수7．8,예측득도화색감적최대제취솔위58．83±1．78％．재최우조건하,화색감적제취솔위58．64±0．32％,상대오차부위0．95％,모형준학도고．초성보조제취비전통매법제취적화색감제취솔고12．96％．경 HPLC 분석,초성보조제취제고료화색감조분적제취솔．
To investigate the extraction rate of anthocyanins,individual variables experiment and Box-Be-hnken design were used to optimize the process parameters of ultrasonic-assisted extraction of anthocya-nins from blueberry pulp.And extraction rate and absorption spectra of anthocyanins obtained under the optimal ultrasonic-assisted extraction were compared with those extracted by traditional enzymatic and standing methods,and the effect of the three extraction methods on the compositions of anthocyanin was analyzed by HPLC.It was found that the optimal extraction parameters were as follows:extraction time 1 1 9 min,extraction temperature 69.7 ℃,ultrasonic time 45.8 min and dilution coefficient 7.8.Un-der this condition,the predictive yield of the anthocyanins from blueberry pulp was 58.83±1.78% and it had a 0.95% relative error to the predictive yield,and the mathematical model was accurate.In addition, the yield obtained by ultrasonic-assisted extraction was 12.96% higher than the yield obtained by tradi-tional enzymatic extraction.The data obtained by HPLC showed that the optimization process had im-proved the yield of the anthocyanins with no significant change in its composition.