CAJ | 학술논문

目的：研究磷酸甘油酸激酶1（ PGK1）对胶质瘤U251细胞放射敏感性的影响。方法建立放射抵抗的U251（RR-U251）细胞；LipofectamineTM2000方法将抑制PGK1表达的shRNA-PGK1质粒或对照shRNA-NC转染至U251细胞；荧光定量PCR及Western Blot检测PGK1的表达；MTT检测细胞相对存活率，划痕实验检测细胞迁移能力，Transwell小室实验检测细胞侵袭能力。结果与正常U251细胞相比，RR-U251细胞中PGK1的表达明显增高；放射处理后，RR-U251细胞的相对存活率升高，迁移能力及侵袭能力增强。转染shRNA-PGK1的细胞接受放射处理后，与对照组相比，细胞的相对存活率降低，迁移能力以及侵袭能力减弱。结论下调U251细胞中PGK1的表达可增加其放射敏感性，提示PGK1可能成为增强放射敏感性的调控靶点。
목적：연구린산감유산격매1（ PGK1）대효질류U251세포방사민감성적영향。방법건립방사저항적U251（RR-U251）세포；LipofectamineTM2000방법장억제PGK1표체적shRNA-PGK1질립혹대조shRNA-NC전염지U251세포；형광정량PCR급Western Blot검측PGK1적표체；MTT검측세포상대존활솔，화흔실험검측세포천이능력，Transwell소실실험검측세포침습능력。결과여정상U251세포상비，RR-U251세포중PGK1적표체명현증고；방사처리후，RR-U251세포적상대존활솔승고，천이능력급침습능력증강。전염shRNA-PGK1적세포접수방사처리후，여대조조상비，세포적상대존활솔강저，천이능력이급침습능력감약。결론하조U251세포중PGK1적표체가증가기방사민감성，제시PGK1가능성위증강방사민감성적조공파점。
Objective To explore the role of phosphoglycerate kinase 1 ( PGK1 ) in the radiosensitivity of glioma U251 cells.Methods Radioresistant U251 cells ( RR-U251 ) were established .The shRNA for PGK1 and the control shRNA were transfected to U 251 cells using lipofectamineTM2000, and real-time quantitative fluorescent PCR and western blot analysis were performed to evaluate PGK1 expression .The cell viability was determined by MTT assay .The wound-healing assay ( WHA) method was used to evaluate cell migration ability .Cell invasion abilities were examined using Transwell culture chamber system .Results Our results showed that the expression of PGK1 was significantly increased in RR-U251 cells compared with normal U251 cells.After irradiation , the cell viability was significantly higher in RR-U251 cells compared with normal U 251 cells, and migration ability and invasion ability was also enhanced .Down-regulating PGK1 using shRNA could induce a significantly down-regulated cell viability ,and decreased the ability of migration and invasion.Conclusion These findings suggest that down-regulation of PGK1 could enhance radiosensitivity in human U 251 cells,and PGK1 may serves as a target for enhancing radiosensitivity .