CAJ | 학술논문

采用选择性培养基从梅河口市某地下水样品中分离出一株对铁、锰有高效去除作用的菌株，将其命名为M HK-10。16S rDNA测序表明，该菌株为芽孢杆菌属（Bacillus），同源性达96％。通过影响因素实验探讨了温度、pH值及地下水中常见阳离子对M H K-10除锰作用的影响。结果表明：M H K-10除锰的最佳条件为温度15～30℃，p H值6～8，且Na＋、Ca2＋的存在对锰离子的去除起促进作用，K＋、Mg2＋、NH＋4、Fe2＋对其起抑制作用。将菌种用于处理所取水样，15 min后锰的去除率达96％以上，出水锰含量达到国家饮用水标准（GB5749-2006），验证了M HK-10除锰的可行性。
채용선택성배양기종매하구시모지하수양품중분리출일주대철、맹유고효거제작용적균주，장기명명위M HK-10。16S rDNA측서표명，해균주위아포간균속（Bacillus），동원성체96％。통과영향인소실험탐토료온도、pH치급지하수중상견양리자대M H K-10제맹작용적영향。결과표명：M H K-10제맹적최가조건위온도15～30℃，p H치6～8，차Na＋、Ca2＋적존재대맹리자적거제기촉진작용，K＋、Mg2＋、NH＋4、Fe2＋대기기억제작용。장균충용우처리소취수양，15 min후맹적거제솔체96％이상，출수맹함량체도국가음용수표준（GB5749-2006），험증료M HK-10제맹적가행성。
A manganese and iron-resistant strain is isolated from groundwater sampling from Meihekou City by selective media ,which is named MHK-10 .The result of 16S rDNA sequencing shows that the MHK-10 belongs to Bacillus .Based on the water quality of field site ,the effect of environmental factors on the removal of manganese are investigated by batch experiment ,temperature ,pH , common cations included .The results indicate that the optimal condition for the bio-oxidation of manganese is as follows :the tem-perature is 15~30 ℃ ,pH value is 6~8 ,the existence of Na+ ,Ca2+ plays a positive role ,while K+ ,Mg2+ ,NH+4 ,Fe2+ plays a nega-tive role .The strain is used for groundwater treatment ,the removal rate reaches above 96% after 15 min ,and manganese content meets standards for drinking water quality (GB5749-2006) ,which proves further the feasibility of the technology .