CAJ | 학술논문

目的：在帕金森病体外模型的多巴胺能神经元中，探讨KLF6的变化及意义。方法用100uM 的MPP＋处理多巴胺能细胞系SN4741细胞，制造帕金森病体外模型。用实时定量PCR和western blot检测KLF6的mRNA以及蛋白水平的变化。通过慢病毒感染在 SN4741细胞中过表达 KLF6，然后用 western blot 检测病毒感染后 KLF6的表达量。在SN4741细胞中过表达KLF6后，用100uM 的MPP＋处理对照组和过表达KLF6组的细胞，通过流式细胞仪和MTT检测细胞凋亡情况。结果（1）和对照组相比，MPP＋处理后的SN4741细胞中KLF6 mRNA和蛋白水平的表达量显著下降（P＜0.05）。（2）过表达KLF6的病毒感染SN4741细胞后，KLF6的蛋白水平显著增加（P＜0.05）。（3）用100 uM 的MPP＋处理对照组和过表达KLF6组，和对照组相比，KLF6过表达组的细胞凋亡比例显著下降（P＜0.05）。结论 KLF6在帕金森病体外模型中起到保护多巴胺能神经元的作用，促进神经元存活，为治疗帕金森病提供了新的靶点和思路。
목적：재파금삼병체외모형적다파알능신경원중，탐토KLF6적변화급의의。방법용100uM 적MPP＋처리다파알능세포계SN4741세포，제조파금삼병체외모형。용실시정량PCR화western blot검측KLF6적mRNA이급단백수평적변화。통과만병독감염재 SN4741세포중과표체 KLF6，연후용 western blot 검측병독감염후 KLF6적표체량。재SN4741세포중과표체KLF6후，용100uM 적MPP＋처리대조조화과표체KLF6조적세포，통과류식세포의화MTT검측세포조망정황。결과（1）화대조조상비，MPP＋처리후적SN4741세포중KLF6 mRNA화단백수평적표체량현저하강（P＜0.05）。（2）과표체KLF6적병독감염SN4741세포후，KLF6적단백수평현저증가（P＜0.05）。（3）용100 uM 적MPP＋처리대조조화과표체KLF6조，화대조조상비，KLF6과표체조적세포조망비례현저하강（P＜0.05）。결론 KLF6재파금삼병체외모형중기도보호다파알능신경원적작용，촉진신경원존활，위치료파금삼병제공료신적파점화사로。
Objective To explore the change of KLF6 and its significance in the dopaminergic neurons of the Parkinson's disease in vitro model. Methods In vitro model of Parkinson's disease were made by treating SN4741 cells with 100 μM MPP+ .Then qRT-PCR and western blot were used to examine the change of KLF6 mRNA and its protein level. After that ,lenti-virus were used to make KLF6 over-express in SN4741 cells. After lentivirus infection ,KLF6 protein level was examined with western blot. Finally ,Flow cytometry and MTT were used to to examine cell apoptosis. Results Compared with the control group ,KLF6 mRNA and protein level were significantly decreased in the MPP + treated group (P<0.05). Lentivirus infec-tion led to a significant increase of KLF6 protein level in SN4741 cell.Up-regulated KLF6 obviously protected SN4741 cells from MPP+. Conclusion KLF6 can protect dopaminergic neurons from MPP+ ,which will provide a new target for the treat-ment of Parkinson's disease.