功能材料
功能材料
공능재료
JOURNAL OF FUNCTIONAL MATERIALS
2014年
8期
8102-8107
,共6页
殷丽华%王红%张忠芮%黄嵩涛%王家奎%秦子顺%孙琪%余占海
慇麗華%王紅%張忠芮%黃嵩濤%王傢奎%秦子順%孫琪%餘佔海
은려화%왕홍%장충예%황숭도%왕가규%진자순%손기%여점해
静电纺丝%复合三维纳米支架%聚左旋乳酸己内酯%理化性能%生物学性能
靜電紡絲%複閤三維納米支架%聚左鏇乳痠己內酯%理化性能%生物學性能
정전방사%복합삼유납미지가%취좌선유산기내지%이화성능%생물학성능
electrospinning%three-dimensiona blending compositenanofibers%polylactic acid%physicochemical properties%biological properties
采用静电纺丝法以六氟异丙醇(HFIP)为溶剂,将质量比为70∶30的 SF/COL (丝素/胶原)和PLLC(聚左旋乳酸己内酯)共混制备质量比为0∶100,30∶70,50∶50,70∶30,100∶0的8% SF/COL/PLCL的三维纳米纤维支架,25%戊二醛蒸汽交联48 h后检测各组比例支架材料的理化性能及生物性能。扫描电镜显示纳米纤维形貌良好,均为纳米级别;X射线衍射结果显示交联后材料结晶度增强,Silk Ⅰ构象由α-折叠形成稳定的β-折叠;材料交联后为疏水性, PLCL的加入明显改善材料的亲水性能;热稳定性随着PLCL浓度的增加也愈加稳定,交联后尤其明显。细胞培养实验证明,随着培养时间延长,细胞在不同比例的支架上粘附、铺展和增殖均良好,但 hPDLSCs 与配比为30∶70的 SF/COL/PLCL 支架复合后增殖速率最快。
採用靜電紡絲法以六氟異丙醇(HFIP)為溶劑,將質量比為70∶30的 SF/COL (絲素/膠原)和PLLC(聚左鏇乳痠己內酯)共混製備質量比為0∶100,30∶70,50∶50,70∶30,100∶0的8% SF/COL/PLCL的三維納米纖維支架,25%戊二醛蒸汽交聯48 h後檢測各組比例支架材料的理化性能及生物性能。掃描電鏡顯示納米纖維形貌良好,均為納米級彆;X射線衍射結果顯示交聯後材料結晶度增彊,Silk Ⅰ構象由α-摺疊形成穩定的β-摺疊;材料交聯後為疏水性, PLCL的加入明顯改善材料的親水性能;熱穩定性隨著PLCL濃度的增加也愈加穩定,交聯後尤其明顯。細胞培養實驗證明,隨著培養時間延長,細胞在不同比例的支架上粘附、鋪展和增殖均良好,但 hPDLSCs 與配比為30∶70的 SF/COL/PLCL 支架複閤後增殖速率最快。
채용정전방사법이륙불이병순(HFIP)위용제,장질량비위70∶30적 SF/COL (사소/효원)화PLLC(취좌선유산기내지)공혼제비질량비위0∶100,30∶70,50∶50,70∶30,100∶0적8% SF/COL/PLCL적삼유납미섬유지가,25%무이철증기교련48 h후검측각조비례지가재료적이화성능급생물성능。소묘전경현시납미섬유형모량호,균위납미급별;X사선연사결과현시교련후재료결정도증강,Silk Ⅰ구상유α-절첩형성은정적β-절첩;재료교련후위소수성, PLCL적가입명현개선재료적친수성능;열은정성수착PLCL농도적증가야유가은정,교련후우기명현。세포배양실험증명,수착배양시간연장,세포재불동비례적지가상점부、포전화증식균량호,단 hPDLSCs 여배비위30∶70적 SF/COL/PLCL 지가복합후증식속솔최쾌。
The aim of this study was tostructure a kind of three-dimensiona nanofiber scaffold composed of SF, collagenⅠand PLCL by using electrospinning technique and to evaluate its performance.We took 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP)as solvent to prepare different mass ratios of SF/collagenⅠ/PLCL scaffolds (0∶100,30∶70,50∶50,70∶30,100∶0)and the properties of these scaffolds were studied before and after they were crosslinked by 25% glutaraldehyde vapor for 48 hours.The results were described as follow:scan-ning electron microscopy(SEM)and the contact water angle meter analysis showed that the uniformity of com-posite nanofibers was improved and with the existence of PLCL,the hydrophilic property decreased.XRD anal-ysis indicates that the nanofibers scaffolds had higher crystallinity after crosslinked.The thermal stability of the scaffold material was also increased.With rising levels of PLCL,the properties of the scaffolds were increased significantly after crosslinking.When the human periodontal ligament stem cells(hPDLSCs)were seeded on SF/collagenⅠ/PLCL scaffolds,they grew well and showed better ability of adhesion and proliferation.Among the scaffolds,PLCL content of 70% did best with significant differences.Our study indicated that this kind of electrospun three-dimensiona nanofiber scaffold might be a potential candidate for restoration of periodontal de-fect and the periodontal tissue engineering.