CAJ | 학술논문

目的：研究脑肠肽对小鼠酒精性肝损伤的保护作用。方法采用4周5%酒精饲料喂饲加5 g·kg-1的高剂量酒精灌胃诱导酒精性肝损伤模型，用分光光度法检测血清中ALT、AST和肝匀浆MDA、SOD、GSH-Px含量；Real-time PCR法检测肝脏中炎症细胞因子 TNF-α、IL-1β、IFN-γ、IL-6和MCP-1的表达。 HE染色检测肝脏病理改变。结果这种慢性酒精喂饲+单剂量大剂量酒精灌胃模式可明显诱导酒精性肝损伤，可导致明显的转氨酶升高、脂肪肝和炎症浸润。给予脑肠肽(5、10、20μg·kg-1) ip后可明显降低酒精性肝损伤小鼠增高的血清ALT、AST活性，改善酒精肝病理改变和炎症浸润；并能减少肝匀浆MDA含量，使降低的肝匀浆SOD活性升高；降低酒精性肝损伤小鼠肝脏的炎症细胞因子TNF-α、IL-1β、IFN-γ、IL-6和MCP-1的mRNA表达。结论脑肠肽对小鼠酒精性肝损伤具保护作用，其机制与抗氧化和抑制炎症作用有关。
목적：연구뇌장태대소서주정성간손상적보호작용。방법채용4주5%주정사료위사가5 g·kg-1적고제량주정관위유도주정성간손상모형，용분광광도법검측혈청중ALT、AST화간균장MDA、SOD、GSH-Px함량；Real-time PCR법검측간장중염증세포인자 TNF-α、IL-1β、IFN-γ、IL-6화MCP-1적표체。 HE염색검측간장병리개변。결과저충만성주정위사+단제량대제량주정관위모식가명현유도주정성간손상，가도치명현적전안매승고、지방간화염증침윤。급여뇌장태(5、10、20μg·kg-1) ip후가명현강저주정성간손상소서증고적혈청ALT、AST활성，개선주정간병리개변화염증침윤；병능감소간균장MDA함량，사강저적간균장SOD활성승고；강저주정성간손상소서간장적염증세포인자TNF-α、IL-1β、IFN-γ、IL-6화MCP-1적mRNA표체。결론뇌장태대소서주정성간손상구보호작용，기궤제여항양화화억제염증작용유관。
Aim To investigate the effects of ghrelin on alcohol-induced liver injury. Methods The alcoholic liver injury mouse model was induced by chronic etha-nol feeding ( 4-week ad libitum oral feeding with the ethanol liquid diet) plus a single binge ethanol (5 g· kg-1 ) feeding. The level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) in serum, malondiadehyde ( MDA ) content, superoxide dis-mutase (SOD) and glutathione peroxidase (GSH-Px) activities in liver homogenate were assayed by spectro-photometer. Hepatic pathological examination was ob-served by HE staining. The mRNA expression of proin-flammatory cytokines including TNF-α, IL-1β, IFN-γ, IL-6 and MCP-1 in the liver was measured by real-time PCR method. Results This chronic-plus-single-binge high dose ethanol feeding synergistically induced liver injury, inflammation and fatty liver change. Treatment with Ghrelin ( 5 , 10 , 20 μg · kg-1 ) significantly de-creased the enhanced level of transaminase ( ALT, AST) in serum, improved the pathologic change in liv-er, and reduced the infiltration of inflammatory cells induced by alcohol administration. Ghrelin also de-creased MDA content and increased the reduced SOD and GSH-Px level in liver homogenate. Furthermore, ghrelin decreased inflammatory cytokines mRNA ex-pression including TNF-α, IL-1β, IFN-γ, IL-6 and MCP-1 in the liver. Conclusion Ghrelin has protec-tive effects against alcoholic liver injury in mice via in-hibiting inflammation and suppressing oxidative stress.