中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2014年
10期
1425-1429,1430
,共6页
内皮细胞疫苗%抗血管生成%OK432%肿瘤免疫治疗%EAC乳腺癌%ICR小鼠
內皮細胞疫苗%抗血管生成%OK432%腫瘤免疫治療%EAC乳腺癌%ICR小鼠
내피세포역묘%항혈관생성%OK432%종류면역치료%EAC유선암%ICR소서
umbilical vein endothelial cell vaccine%anti-angiogenesis therapy%OK432%cancer immuno-therapy%EAC breast cancer%ICR mice
目的：探讨 OK432优化的人脐静脉内皮细胞( HU-VECs)疫苗对小鼠EAC乳腺癌的生长抑制作用。方法体外培养HUVECs,与佐剂OK432混合,制备HUVECs-OK432疫苗,以小鼠 EAC 乳腺癌皮下移植瘤模型考查 HUVECs-OK432疫苗的抗肿瘤效应,并通过ELISA、脾细胞增殖及细胞毒性T淋巴细胞( CTL)杀伤实验检测疫苗免疫后体液及细胞免疫应答水平。结果在预防性免疫中, HUVECs-OK432疫苗可以明显抑制EAC乳腺癌生长；HUVECs-OK432疫苗诱导小鼠产生了高滴度的特异性 HUVEC 抗体；HU-VECs-OK432疫苗能有效刺激免疫小鼠脾淋巴细胞的增殖；HUVECs-OK432组小鼠脾细胞诱导产生了明显的靶向HU-VEC细胞的CTL杀伤作用。结论 HUVECs-OK432疫苗可以有效诱导机体产生靶向HUVEC的特异性体液及细胞免疫应答,从而有效抑制了小鼠EAC乳腺癌的生长。
目的：探討 OK432優化的人臍靜脈內皮細胞( HU-VECs)疫苗對小鼠EAC乳腺癌的生長抑製作用。方法體外培養HUVECs,與佐劑OK432混閤,製備HUVECs-OK432疫苗,以小鼠 EAC 乳腺癌皮下移植瘤模型攷查 HUVECs-OK432疫苗的抗腫瘤效應,併通過ELISA、脾細胞增殖及細胞毒性T淋巴細胞( CTL)殺傷實驗檢測疫苗免疫後體液及細胞免疫應答水平。結果在預防性免疫中, HUVECs-OK432疫苗可以明顯抑製EAC乳腺癌生長；HUVECs-OK432疫苗誘導小鼠產生瞭高滴度的特異性 HUVEC 抗體；HU-VECs-OK432疫苗能有效刺激免疫小鼠脾淋巴細胞的增殖；HUVECs-OK432組小鼠脾細胞誘導產生瞭明顯的靶嚮HU-VEC細胞的CTL殺傷作用。結論 HUVECs-OK432疫苗可以有效誘導機體產生靶嚮HUVEC的特異性體液及細胞免疫應答,從而有效抑製瞭小鼠EAC乳腺癌的生長。
목적：탐토 OK432우화적인제정맥내피세포( HU-VECs)역묘대소서EAC유선암적생장억제작용。방법체외배양HUVECs,여좌제OK432혼합,제비HUVECs-OK432역묘,이소서 EAC 유선암피하이식류모형고사 HUVECs-OK432역묘적항종류효응,병통과ELISA、비세포증식급세포독성T림파세포( CTL)살상실험검측역묘면역후체액급세포면역응답수평。결과재예방성면역중, HUVECs-OK432역묘가이명현억제EAC유선암생장；HUVECs-OK432역묘유도소서산생료고적도적특이성 HUVEC 항체；HU-VECs-OK432역묘능유효자격면역소서비림파세포적증식；HUVECs-OK432조소서비세포유도산생료명현적파향HU-VEC세포적CTL살상작용。결론 HUVECs-OK432역묘가이유효유도궤체산생파향HUVEC적특이성체액급세포면역응답,종이유효억제료소서EAC유선암적생장。
Aim To investigate the anti-EAC breast cancer effects of viable human umbilical vein endothe-lial cells ( HUVECs) vaccine. Methods Subconflu-ent HUVECs were mixed with OK432 to prepare HU-VECs-OK432 vaccine, and the anti-tumor efficacy of HUVECs-OK432 was investigated using a subcutaneous tumor model of EAC breast cancer. ELISA, splenic lymphocyte proliferation assay and cytotoxic T lympho-cytes ( CTL ) killing assay were adopted to detect the humoral and cellular immune responses after HUVECs-OK432 immunization. Results HUVECs-OK432 im-munization significantly inhibited the growth of EAC tumor in mice in the prophylactic procedures. High ti-ter of anti-HUVEC antibody was elicited by HUVECs-OK432 immunization. The proliferation activity of splenocytes from mice immunized with HUVECs-OK432 was significantly increased. T lymphocytes iso-lated from HUVECs-OK432-immunized mice were dose dependently killing HUVECs in vitro. Conclusion Strong humoral and cellular immune responses targeting HUVEC are elicited by HUVECs-OK432 immuniza-tion, which results in a significant inhibition on the growth of EAC tumor in mice.
