CAJ | 학술논문

目的：研究槲皮素联合顺铂对人骨肉瘤MG-63细胞增殖及凋亡的影响，并初步探讨其作用机制。方法采用不同浓度槲皮素与顺铂单独及联合作用MG-63细胞；倒置相差显微镜观察细胞形态变化；CCK-8法检测细胞增殖抑制率，并通过Chou-Talaly联合指数法分析两药之间相互作用；流式细胞仪检测细胞凋亡率；RT-PCR 及 Western blot 检测Bcl-2、caspase-3等凋亡相关分子在基因及蛋白水平的表达变化。结果槲皮素及顺铂均能抑制MG-63细胞增殖并诱导其凋亡，两药联合具有协同效应，并可下调Bcl-2及上调caspase-3基因及蛋白的表达。结论槲皮素联合顺铂能协同抑制MG-63细胞增殖并诱导其凋亡，其作用机制可能与下调Bcl-2及上调caspase-3等凋亡相关分子的表达有关。
목적：연구곡피소연합순박대인골육류MG-63세포증식급조망적영향，병초보탐토기작용궤제。방법채용불동농도곡피소여순박단독급연합작용MG-63세포；도치상차현미경관찰세포형태변화；CCK-8법검측세포증식억제솔，병통과Chou-Talaly연합지수법분석량약지간상호작용；류식세포의검측세포조망솔；RT-PCR 급 Western blot 검측Bcl-2、caspase-3등조망상관분자재기인급단백수평적표체변화。결과곡피소급순박균능억제MG-63세포증식병유도기조망，량약연합구유협동효응，병가하조Bcl-2급상조caspase-3기인급단백적표체。결론곡피소연합순박능협동억제MG-63세포증식병유도기조망，기작용궤제가능여하조Bcl-2급상조caspase-3등조망상관분자적표체유관。
Aim To investigate the effect and mecha-nism of quercetin combined with cisplatin on prolifera-tion and apoptosis of human osteosarcoma cell line MG-63 . Methods MG-63 cells were treated with quercetin alone or combined with cisplatin. Cellular morphologic changes were observed under inverted phase contrast microscope. The effects of proliferation inhibition were assayed by CCK-8 method. The combination effect was judged through Chou-Talaly analysis. The apoptosis ra-tios of cells were analyzed by flow cytometry. The gene expression of Bcl-2 and caspase-3 was detected by RT-PCR assay. The protein expression of Bcl-2 and caspase-3 was measured by Western blot assay. Re-sults Quercetin alone or combined with cisplatin could inhibit the proliferation, but induce the apoptosis of MG-63 cells. Combination of quercetin and cisplatin revealed a synergistic effect on cell proliferation and apoptosis as it reduced the expression of Bcl-2 but en-hanced that of caspase-3 at both gene and protein lev-els. Conclusion Synergistic effect of quercetin com-bined with cisplatin on cell proliferation and apoptosis of MG-63 cells is possibly due to reduction of Bcl-2 and enhancement of caspase-3 expression.