CAJ | 학술논문

目的：探讨TP对肿瘤相关的趋化因子巨噬细胞炎性蛋白1-α( MIP-1α)表达的影响。方法使用RT-PCR的试验方法,检测小鼠巨噬细胞Ana-1细胞和肿瘤组织中提取的巨噬细胞中MIP-1α的表达；建立小鼠S180肉瘤细胞皮下移植瘤双瘤模型,待肿瘤体积生长至约250 mm3时,将其中一侧建立术后残瘤模型,并开始以8 mg·kg-1剂量的TP给药,隔天1次,给药4次后,分离肿瘤组织,免疫组化检测MIP-1α的表达。结果不同浓度TP对小鼠TAMs的MIP-1α表达较空白对照组明显减少,并呈剂量依赖性；体内试验中,TP处理组的荷瘤小鼠的肿瘤组织MIP-1α与对照组相比表达明显降低,接近于阳性药环磷酰胺组；但是 MIP-1α在小鼠Ana-1细胞和TAMs中的 mRNA表达量差异没有统计学意义；不同浓度TP对小鼠Ana-1细胞的MIP-1α表达差异也没有统计学意义。结论 TP 不能影响小鼠 Ana-1细胞系的MIP-1α表达,但TP对肿瘤组织中的巨噬细胞MIP-1α的表达有明显影响；MIP-1α有可能是 TP抗癌作用机制的新靶点。
목적：탐토TP대종류상관적추화인자거서세포염성단백1-α( MIP-1α)표체적영향。방법사용RT-PCR적시험방법,검측소서거서세포Ana-1세포화종류조직중제취적거서세포중MIP-1α적표체；건립소서S180육류세포피하이식류쌍류모형,대종류체적생장지약250 mm3시,장기중일측건립술후잔류모형,병개시이8 mg·kg-1제량적TP급약,격천1차,급약4차후,분리종류조직,면역조화검측MIP-1α적표체。결과불동농도TP대소서TAMs적MIP-1α표체교공백대조조명현감소,병정제량의뢰성；체내시험중,TP처리조적하류소서적종류조직MIP-1α여대조조상비표체명현강저,접근우양성약배린선알조；단시 MIP-1α재소서Ana-1세포화TAMs중적 mRNA표체량차이몰유통계학의의；불동농도TP대소서Ana-1세포적MIP-1α표체차이야몰유통계학의의。결론 TP 불능영향소서 Ana-1세포계적MIP-1α표체,단TP대종류조직중적거서세포MIP-1α적표체유명현영향；MIP-1α유가능시 TP항암작용궤제적신파점。
Aim To investigate the effect of TP on the expression of macrophages inflammatory protein ( MIP-1α) . Methods Total RNA of mouse Ana-1 cells and tumor associated macrophages were extracted, and MIP-1α mRNA was detected by RT-PCR. Mouse S180-xenografts were established by injecting S180 cells subcutaneously into the double abdominal flanks of the mice. The postoperative residual tumor models were generated in the right abdominal tumors when tumors grew into 250 mm3 . Animals were treated with TP or CTX, and tumor tissues were separated and MIP-1α was detected by immunohistochemistry. Results There was no significant difference of the expression of MIP-1α between Ana-1 cells and TAMs. TP couldn’ t affect MIP-1αexpression in Ana-1 cells while it signifi-cantly decrease MIP-1α expression in TAMs in a dose-dependent manner. TP significantly decreased MIP-1αexpression of tumor tissue compared with control group. Conclusions MIP-1α will be a new target of TP anti-cancer. Simple cell line tests in vitro couldn’ t reveal the real state in vivo.