CAJ | 학술논문

建立高效液相色谱-串联四极杆质谱正负离子切换模式同时快速测定黄鱼中黄色工业染料碱性嫩黄O及酸性橙Ⅱ的检测方法。样品匀质后，经乙腈提取，盐析，-20℃冷冻脱脂，低温离心后，取乙腈层采用高效液相色谱-串联质谱法分离、测定。结果表明碱性嫩黄O在0.5滋g/kg~10滋g/kg、酸性橙Ⅱ在5滋g/kg~100滋g/kg范围内呈良好的线性关系，高中低浓度回收率在70%~110%，相对标准偏差（n=5）小于15%，碱性嫩黄O、酸性橙Ⅱ检出限分别为0.5、5滋g/kg。本方法适用于黄鱼中违禁工业染料碱性嫩黄O及酸性橙Ⅱ的测定。
건립고효액상색보-천련사겁간질보정부리자절환모식동시쾌속측정황어중황색공업염료감성눈황O급산성등Ⅱ적검측방법。양품균질후，경을정제취，염석，-20℃냉동탈지，저온리심후，취을정층채용고효액상색보-천련질보법분리、측정。결과표명감성눈황O재0.5자g/kg~10자g/kg、산성등Ⅱ재5자g/kg~100자g/kg범위내정량호적선성관계，고중저농도회수솔재70%~110%，상대표준편차（n=5）소우15%，감성눈황O、산성등Ⅱ검출한분별위0.5、5자g/kg。본방법괄용우황어중위금공업염료감성눈황O급산성등Ⅱ적측정。
A high performance liquid chromatography tandem quadrupole mass spectrometry method was developed for rapid determination of Auramine O and Acid orangeⅡin yellow croaker. The sample was extracted by acetonitrile, the fat was removed through freezing at-20℃, then analyzed by LC-MS/MS. The results showed good linearity in the ranges of 0.5 μg/kg-10μg/kg,5μg/kg-100μg/kg for Auramine O and Acid orangeⅡ, respectively. The recoveries were between 70%and 110%, and the value of the RSD (n=5) was lower than 15%for the both. The limit of quantitative (LOQ) for Auramine O and Acid orangeⅡwas 0.5μg/kg and 5μg/kg, separately. The method was suitable to detect Auramine O and Acid orangeⅡas two banned industrial dyes in yellow croaker.