CAJ | 학술논문

植物核苷二磷酸激酶（NDPKs），属于蛋白激酶家族，参与植物的多种生理生化过程，在植物响应非生物胁迫过程中发挥重要作用。NDPK2基因转化紫花苜蓿，对提高紫花苜蓿的耐逆性具有重要意义。试验以“敖汉”苜蓿下胚轴的再生系统为基础，通过农杆菌介导法将SWPA2驱动的AtNDPK2基因转入紫花苜蓿下胚轴，获得抗性植株。通过PCR初步检测证明该基因已经整合到“敖汉”苜蓿的基因组，阳性植株转化率为23．3％。不同浓度NaCl胁迫测定转基因植株的SOD、POD活性及质膜相对电导率、MDA含量，结果发现在0．4％～1．0％NaCl胁迫下转基因植株的SOD、POD酶活性显著高于对照，相对电导率、MDA含量显著的低于对照，进一步说明AtNDPK2基因的转入增强了“敖汉”苜蓿的耐盐性。
식물핵감이린산격매（NDPKs），속우단백격매가족，삼여식물적다충생리생화과정，재식물향응비생물협박과정중발휘중요작용。NDPK2기인전화자화목숙，대제고자화목숙적내역성구유중요의의。시험이“오한”목숙하배축적재생계통위기출，통과농간균개도법장SWPA2구동적AtNDPK2기인전입자화목숙하배축，획득항성식주。통과PCR초보검측증명해기인이경정합도“오한”목숙적기인조，양성식주전화솔위23．3％。불동농도NaCl협박측정전기인식주적SOD、POD활성급질막상대전도솔、MDA함량，결과발현재0．4％～1．0％NaCl협박하전기인식주적SOD、POD매활성현저고우대조，상대전도솔、MDA함량현저적저우대조，진일보설명AtNDPK2기인적전입증강료“오한”목숙적내염성。
Nucleoside double phosphate kinase (NDPKs)belongs to the family of protein kinases,participating in physiological and biochemical processes in plants,playing an important role in the process of the abiotic stress.Transfor-mation of alfalfa NDPK2 gene would improve the resistance of alfalfa to play an important significance in reverse.This research was based on alfalfa hypocotyls regeneration system of “Aohan”,mediated by Agrobacterium tumefaciens to transform AtNDPK2 gene driven by SWPA2 into Alfalfa hypocotyls,get resistant plants.The result of PCR preliminary analysis showed that the gene had been integrated into the alfalfa genome of“Aohan”,positive plant transformation rate was 23.3%.The results on testing of transgenic plant SOD,POD activity and membrane relative conductivity and MDA under different concentrations of NaCl stress,showed that SOD,POD enzyme activity of transgenic plants under 0.4%~1 .0%NaCl stress was significantly higher than that of control;The relative conductivity and MDA content was signifi-cantly lower than that of control,further showed that AtNDPK2 cDNA increased the Salt-tolerance of“Aohan”alfalfa.