激光生物学报
激光生物學報
격광생물학보
ACTA LASER BIOLOGY SINICA
2014年
1期
45-50
,共6页
陈瑶%代剑华%张华%周学谦%刘云杰%彭贵勇
陳瑤%代劍華%張華%週學謙%劉雲傑%彭貴勇
진요%대검화%장화%주학겸%류운걸%팽귀용
胃粘膜%胃癌%拉曼光谱%拉曼位移
胃粘膜%胃癌%拉曼光譜%拉曼位移
위점막%위암%랍만광보%랍만위이
gastric mucosa%gastric cancer%raman spectroscopy%raman shift
目的：分析研究胃正常和癌变粘膜组织的拉曼光谱特征，为拉曼光谱应用于胃癌的临床检测诊断奠定基础。方法：收集胃镜检查中活检的19例正常和12例癌变胃粘膜组织标本，采用785 nm激发光拉曼光谱仪进行拉曼光谱采集。比较分析胃正常和癌变粘膜组织的拉曼光谱特征差异并研究其区分正常和癌变组织的价值。结果：1）特征峰1098 cm-1、1444 cm-1、1555 cm-1、1660 cm-1等在胃癌组织中发生了移位，平均位移（2．57±1．28）cm-1，以红移为主；2）癌变组织中相对峰强比I1087 cm-1/I1207 cm-1≥1．87，其区别胃癌和正常胃粘膜组织的准确率、灵敏度和特异度分别为87．1％、83．3％、89．5％；3）癌组织中增加了表征蛋白质的特征峰1262 cm-1、1586 cm-1，但同时减少了表征蛋白质和脂质特征峰1172 cm-1。结论：拉曼光谱不仅可以准确区分正常和癌变，而且可以探索癌变相关的分子生化改变。拉曼光谱在胃癌的跟踪发现和检测诊断中具有良好前景。
目的：分析研究胃正常和癌變粘膜組織的拉曼光譜特徵，為拉曼光譜應用于胃癌的臨床檢測診斷奠定基礎。方法：收集胃鏡檢查中活檢的19例正常和12例癌變胃粘膜組織標本，採用785 nm激髮光拉曼光譜儀進行拉曼光譜採集。比較分析胃正常和癌變粘膜組織的拉曼光譜特徵差異併研究其區分正常和癌變組織的價值。結果：1）特徵峰1098 cm-1、1444 cm-1、1555 cm-1、1660 cm-1等在胃癌組織中髮生瞭移位，平均位移（2．57±1．28）cm-1，以紅移為主；2）癌變組織中相對峰彊比I1087 cm-1/I1207 cm-1≥1．87，其區彆胃癌和正常胃粘膜組織的準確率、靈敏度和特異度分彆為87．1％、83．3％、89．5％；3）癌組織中增加瞭錶徵蛋白質的特徵峰1262 cm-1、1586 cm-1，但同時減少瞭錶徵蛋白質和脂質特徵峰1172 cm-1。結論：拉曼光譜不僅可以準確區分正常和癌變，而且可以探索癌變相關的分子生化改變。拉曼光譜在胃癌的跟蹤髮現和檢測診斷中具有良好前景。
목적：분석연구위정상화암변점막조직적랍만광보특정，위랍만광보응용우위암적림상검측진단전정기출。방법：수집위경검사중활검적19례정상화12례암변위점막조직표본，채용785 nm격발광랍만광보의진행랍만광보채집。비교분석위정상화암변점막조직적랍만광보특정차이병연구기구분정상화암변조직적개치。결과：1）특정봉1098 cm-1、1444 cm-1、1555 cm-1、1660 cm-1등재위암조직중발생료이위，평균위이（2．57±1．28）cm-1，이홍이위주；2）암변조직중상대봉강비I1087 cm-1/I1207 cm-1≥1．87，기구별위암화정상위점막조직적준학솔、령민도화특이도분별위87．1％、83．3％、89．5％；3）암조직중증가료표정단백질적특정봉1262 cm-1、1586 cm-1，단동시감소료표정단백질화지질특정봉1172 cm-1。결론：랍만광보불부가이준학구분정상화암변，이차가이탐색암변상관적분자생화개변。랍만광보재위암적근종발현화검측진단중구유량호전경。
Object:Raman spectra features of normal and malignant gastric mucosal tissues were analysed to pave the way for Raman Spectroscopy being applied to clinical tumor detection and diagnosis.Methods:A total of 3 1 gastric tis-sues were collected for Raman Spectroscopic measurements with a rapid-acquisition NIR-Raman system at 785-nm exci-tion.With a comparitive study of Raman spectrums aquired respectively from normal and malignant gastric mucosal tis-sues,its potential in discriminating normal from malignant gastric tissues can be further evaluated.Results:1 )Some characteristic peaks shifted in malignant tissues,e.g.peaks at 1 098 cm-1、1 444 cm-1、1 555 cm-1、1 660 cm-1 and so on,which were mainly red shift,and the average shift was about 2.57 ±1.28 cm-1;2)The ratio of relative intensity,I1 087 cm-1/I1 207 cm-1,is not less than 1.87 in gastric tissues,which yielded a diagnosis accuracy of 87.1%,sensitivity of 83 .3% and specificity of 89 .5% respectively;3 )An extra characteristic peak indicating protein appeared at 1 262 cm-1 in cancer tissues,while another characteristic peak indicating lipid and protein at 1 172 cm-1 disappeared.Conclusion:Raman spectroscopy can not only discriminate cancer from normal tissues accurately,but also has great potential in pro-bing biomolecular alteration associated with canceration.Raman spectroscopy has show excellent application prospects in gastric cancer detection and diagnosis.
