CAJ | 학술논문

自噬在细胞的生命过程中起了非常重要的作用，它能通过清除受损的细胞器和过多的蛋白质以维持细胞内环境稳定基本能量代谢的稳定。然而，自噬的持续激活可导致细胞器以及必需的蛋白质的过度消耗，导致不依赖caspase的自噬性细胞死亡。因此，通过这种自噬途径诱导细胞死亡可能是癌症治疗的一种新方法。在本研究我们发现，硫链丝菌素能够减少非小细胞肺癌PC-9细胞的细胞活力和诱导细胞凋亡。另外，我们发现硫链丝菌素诱导了PC-9细胞自噬。此外，我们也发现硫链丝菌素诱导的细胞凋亡可以被自噬抑制剂3-甲基腺嘌呤（3-MA）阻止。这些结果表明，硫链丝菌素促进人体非小细胞肺癌细胞的自噬性死亡。在本研究我们的发现也提示硫链丝菌素联合自噬诱导剂可能在临床上对治疗人非小细胞肺癌有效。
자서재세포적생명과정중기료비상중요적작용，타능통과청제수손적세포기화과다적단백질이유지세포내배경은정기본능량대사적은정。연이，자서적지속격활가도치세포기이급필수적단백질적과도소모，도치불의뢰caspase적자서성세포사망。인차，통과저충자서도경유도세포사망가능시암증치료적일충신방법。재본연구아문발현，류련사균소능구감소비소세포폐암PC-9세포적세포활력화유도세포조망。령외，아문발현류련사균소유도료PC-9세포자서。차외，아문야발현류련사균소유도적세포조망가이피자서억제제3-갑기선표령（3-MA）조지。저사결과표명，류련사균소촉진인체비소세포폐암세포적자서성사망。재본연구아문적발현야제시류련사균소연합자서유도제가능재림상상대치료인비소세포폐암유효。
Autophagy is a fundamental cellular process that controls cells in a normal homeostatic state by recycling nu-trients to maintain cellular energy levels for cell survival through the turnover of proteins and damaged organelles.Where-as,persistent activation of autophagy can cause excessive depletion of cellular organelles and essential proteins,leading to caspase-independent autophagic cell death.Therefore,inducing cell death via this autophagic pathway might be an al-ternative approach for cancer therapy.Here,we show that the thiazole antibiotic thiostrepton decreased cell viability and induced cell apoptosis in non-small-cell lung cancer (NSCLC)PC-9 cells.Additionally,we found thiostrepton induced cell autophagy in PC-9 cells.Moreover,the cell apoptosis induced by thiostrepton could be blocked by 3-Methyladenine (3-MA),a autophagy inhibitor.These findings suggested that thiostrepton can promote the autophagic death of human NSCLC cells.Moreover,this study provided evidence that administration of thiostrepton combined with autophagy induc-er may be an effective therapeutic strategy for targeting human non-small-cell lung cancers.